Histophysiological changes of hypophyseal pars intermedia of rats were investigated in a condition of acute ethanol treatment. 20 male Wistar rats, aged 3 months, were intraperitoneally applicated 15% solution of ethyl alcohol in a different amount and time sacrifice after ethanol treatment (1 g/kg body weight-1 hour; 1 g/kg body weight-3 hours, 3 g/kg body weight-1 hour; 3 g/kg body weight-3 hours). For alcohol analysis blood was collected by method of Widmark at the moment of sacrifice. During the investigation classic light microscopy methods were applicated; then histological analysis was realized and average volume of nuclei glandular cells was monitored; a significance of statistics was showed, too. Changes of glandular cells were in the sense of decreased synthetic activity and inhibition of the secretory process. It was found high significant decreased value of nuclei volume. The hormonal secretion of the intermedial lobe is influenced with transmitter substances. These changes could be interpreted as a result of the increased activities of the inhibitory neurotransmitter dopamine under the influence of ethanol. At the same time acetyl-choline as stimulative factor is decreased.