BIOMAT Database Logo BIOMAT Database Logo

Measurement of cyclosporine concentrations in whole blood: HPLC and radioimmunoassay with a specific monoclonal antibody and 3H- or 125I-labeled ligand compared. 1989

B A Wolf, and M C Daft, and J W Koenig, and M W Flye, and J W Turk, and M G Scott
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.

We compared cyclosporine concentrations in whole blood as measured by HPLC and by RIA with a monoclonal antibody specific for cyclosporine with 3H- or 125I-labeled cyclosporine ligand. The 3H-RIA kit slightly underestimated cyclosporine concentrations (greater than 600 micrograms/L) in comparison with HPLC. Over a wide range of concentrations, cyclosporine measured with the 125I-RIA kit correlated well with HPLC (slope = 0.99, n = 301, r = 0.98), observed for samples from recipients of kidney, heart, or liver allografts (respective slopes: 1.01, 0.93, and 1.00). The 125I-RIA standard curve was linear to 1000 micrograms of cyclosporine per liter. Inter- and intra-assay CVs for 125I-RIA measurements of cyclosporine were less than or equal to 7%. Evidently, the 125I-RIA kit involving a monoclonal antibody specific for cyclosporine is equivalent to the HPLC assay and can replace it for therapeutic drug monitoring of cyclosporine therapy.

UI MeSH Term Description Entries
D007457 Iodine Radioisotopes Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes. Radioisotopes, Iodine
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D012044 Regression Analysis Procedures for finding the mathematical function which best describes the relationship between a dependent variable and one or more independent variables. In linear regression (see LINEAR MODELS) the relationship is constrained to be a straight line and LEAST-SQUARES ANALYSIS is used to determine the best fit. In logistic regression (see LOGISTIC MODELS) the dependent variable is qualitative rather than continuously variable and LIKELIHOOD FUNCTIONS are used to find the best relationship. In multiple regression, the dependent variable is considered to depend on more than a single independent variable. Regression Diagnostics,Statistical Regression,Analysis, Regression,Analyses, Regression,Diagnostics, Regression,Regression Analyses,Regression, Statistical,Regressions, Statistical,Statistical Regressions
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D003524 Cyclosporins A group of closely related cyclic undecapeptides from the fungi Trichoderma polysporum and Cylindocarpon lucidum. They have some antineoplastic and antifungal action and significant immunosuppressive effects. Cyclosporins have been proposed as adjuvants in tissue and organ transplantation to suppress graft rejection. Cyclosporines
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D014316 Tritium The radioactive isotope of hydrogen also known as hydrogen-3. It contains two NEUTRONS and one PROTON in its nucleus and decays to produce low energy BETA PARTICLES. Hydrogen-3,Hydrogen 3
D016027 Heart Transplantation The transference of a heart from one human or animal to another. Cardiac Transplantation,Grafting, Heart,Transplantation, Cardiac,Transplantation, Heart,Cardiac Transplantations,Graftings, Heart,Heart Grafting,Heart Graftings,Heart Transplantations,Transplantations, Cardiac,Transplantations, Heart
D016030 Kidney Transplantation The transference of a kidney from one human or animal to another. Grafting, Kidney,Renal Transplantation,Transplantation, Kidney,Transplantation, Renal,Kidney Grafting,Kidney Transplantations,Renal Transplantations,Transplantations, Kidney,Transplantations, Renal

Related Publications

B A Wolf, and M C Daft, and J W Koenig, and M W Flye, and J W Turk, and M G Scott
Specific 3H radioimmunoassay with a monoclonal antibody for monitoring cyclosporine in blood.
February 1988, Clinical chemistry,
B A Wolf, and M C Daft, and J W Koenig, and M W Flye, and J W Turk, and M G Scott
Measurement of cyclosporine in plasma from patients with various transplants: HPLC radioimmunoassay with a specific monoclonal antibody compared.
August 1989, Clinical chemistry,
B A Wolf, and M C Daft, and J W Koenig, and M W Flye, and J W Turk, and M G Scott
Specific measurement of cyclosporine concentrations in whole blood: radioimmunoassay and fluorescence polarization immunoassay compared.
December 1991, Clinical chemistry,
B A Wolf, and M C Daft, and J W Koenig, and M W Flye, and J W Turk, and M G Scott
Measurement of cyclosporine A by a specific radioimmunoassay with a monoclonal antibody and 125I tracer.
February 1991, Clinical biochemistry,
B A Wolf, and M C Daft, and J W Koenig, and M W Flye, and J W Turk, and M G Scott
Monoclonal antibody fluorescent polarisation immunoassay versus 125Iode labelled ligand radioimmunoassay for the measurement of cyclosporine concentrations in whole blood.
January 1991, Fundamental & clinical pharmacology,
B A Wolf, and M C Daft, and J W Koenig, and M W Flye, and J W Turk, and M G Scott
Determination of cyclosporine in plasma: specific radioimmunoassay with a monoclonal antibody and liquid chromatography compared.
April 1989, Clinical chemistry,
B A Wolf, and M C Daft, and J W Koenig, and M W Flye, and J W Turk, and M G Scott
Radioimmunoassay of salivary cyclosporine with use of 125I-labeled cyclosporine.
August 1988, Clinical chemistry,
B A Wolf, and M C Daft, and J W Koenig, and M W Flye, and J W Turk, and M G Scott
Comparison of cyclosporine determinations in whole blood by three different methods. HPLC, 125I RIA and 3H RIA.
April 1987, American journal of clinical pathology,
B A Wolf, and M C Daft, and J W Koenig, and M W Flye, and J W Turk, and M G Scott
Substitution of 125I-labeled cyclosporine in the radioimmunoassay of plasma cyclosporine.
February 1988, Transplantation,
B A Wolf, and M C Daft, and J W Koenig, and M W Flye, and J W Turk, and M G Scott
Simple and specific isocratic liquid chromatographic procedure for cyclosporine A determination in whole blood, compared with radioimmunoassay.
January 1987, Journal of chromatography,
Copied contents to your clipboard!