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Plasmodium falciparum was shown to synthesize pteroylpolyglutamate de novo from guanosine 5'-triphosphate (GTP), p-aminobenzoate (PABA), and L-glutamate (L-Glu). The parasite also had the capacity to synthesize pteroylpolyglutamate from both intact and degradation moieties (p-aminobenzoylglutamate and pterin-aldehyde) of exogenous folate added into the growth medium. The major product was identified as 5-methyl-tetrahydroteroylpentaglutamate following exposure to pteroylpolyglutamate hydrolase and oxidative degradation of the C9-N10 bond in the molecule and identification of products by reversed-phase high performance liquid chromatography. Inhibition of pteroylpentaglutamate synthesis from the radiolabelled metabolic precursors (GTP, PABA, L-Glu) and folate by the antifolate antimalarials, pyrimethamine and sulfadoxine at therapeutic concentrations, may suggest the existence of a unique biosynthetic pathway in the malaria parasite.
J Krungkrai, and
H K Webster, and
Y Yuthavong
January 2001,
Molecular and biochemical parasitology,
J Krungkrai, and
H K Webster, and
Y Yuthavong
December 1996,
Parasitology today (Personal ed.),
J Krungkrai, and
H K Webster, and
Y Yuthavong
August 2008,
Eukaryotic cell,
J Krungkrai, and
H K Webster, and
Y Yuthavong
May 2004,
Molecular and biochemical parasitology,
J Krungkrai, and
H K Webster, and
Y Yuthavong
September 2006,
Biochemical and biophysical research communications,
J Krungkrai, and
H K Webster, and
Y Yuthavong
April 2011,
Eukaryotic cell,
J Krungkrai, and
H K Webster, and
Y Yuthavong
February 2005,
The Journal of biological chemistry,
J Krungkrai, and
H K Webster, and
Y Yuthavong
June 1999,
International journal for parasitology,
J Krungkrai, and
H K Webster, and
Y Yuthavong
September 2011,
Proceedings of the National Academy of Sciences of the United States of America,
J Krungkrai, and
H K Webster, and
Y Yuthavong
January 2004,
Methods in molecular biology (Clifton, N.J.),
