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Pseudorabies virus (PRV) early protein UL31 is a homologue of herpes simplex virus 1 (HSV-1) UL31, which is a multifunctional protein important for HSV-1 infection. However, the precise roles of PRV UL31 in virus life cycle are still poorly understood. A relatively crucial tool for uncovering the function of UL31 is an antiserum that specifically detects UL31 in the PRV-infected cells. For this purpose, a recombinant UL31 protein consisting of N-terminal 27 aa of UL31 fused to EYFP and His-tag was expressed, purified and used for the preparation of antiserum in BALB/c mice. Our results show that Western blot analysis and immunofluorescence assay showed that this antiserum could specifically detect the purified recombinant UL31 as well as full-length UL31 in the PRV infected cells. These results demonstrate that the prepared antiserum could serve as a valuable tool for further studies of UL31 functions in PRV infection.
M S Cai, and
S Jiang, and
C C Mo, and
J L Wang, and
J L Huang, and
Z C Zeng, and
X W Li, and
Y J Yang, and
C K Chen, and
W Cui, and
M L Li
January 2012,
Acta virologica,
M S Cai, and
S Jiang, and
C C Mo, and
J L Wang, and
J L Huang, and
Z C Zeng, and
X W Li, and
Y J Yang, and
C K Chen, and
W Cui, and
M L Li
March 2014,
Genetics and molecular research : GMR,
M S Cai, and
S Jiang, and
C C Mo, and
J L Wang, and
J L Huang, and
Z C Zeng, and
X W Li, and
Y J Yang, and
C K Chen, and
W Cui, and
M L Li
September 2006,
Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue,
M S Cai, and
S Jiang, and
C C Mo, and
J L Wang, and
J L Huang, and
Z C Zeng, and
X W Li, and
Y J Yang, and
C K Chen, and
W Cui, and
M L Li
February 2003,
Sheng li xue bao : [Acta physiologica Sinica],
M S Cai, and
S Jiang, and
C C Mo, and
J L Wang, and
J L Huang, and
Z C Zeng, and
X W Li, and
Y J Yang, and
C K Chen, and
W Cui, and
M L Li
January 2019,
Iranian journal of biotechnology,
M S Cai, and
S Jiang, and
C C Mo, and
J L Wang, and
J L Huang, and
Z C Zeng, and
X W Li, and
Y J Yang, and
C K Chen, and
W Cui, and
M L Li
August 2012,
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
M S Cai, and
S Jiang, and
C C Mo, and
J L Wang, and
J L Huang, and
Z C Zeng, and
X W Li, and
Y J Yang, and
C K Chen, and
W Cui, and
M L Li
January 2020,
Antiviral research,
M S Cai, and
S Jiang, and
C C Mo, and
J L Wang, and
J L Huang, and
Z C Zeng, and
X W Li, and
Y J Yang, and
C K Chen, and
W Cui, and
M L Li
January 2023,
Frontiers in microbiology,
M S Cai, and
S Jiang, and
C C Mo, and
J L Wang, and
J L Huang, and
Z C Zeng, and
X W Li, and
Y J Yang, and
C K Chen, and
W Cui, and
M L Li
January 1976,
Advances in experimental medicine and biology,
M S Cai, and
S Jiang, and
C C Mo, and
J L Wang, and
J L Huang, and
Z C Zeng, and
X W Li, and
Y J Yang, and
C K Chen, and
W Cui, and
M L Li
April 2005,
Virology,
