Characterization of IL-22 and IL-17 Expressing Leukocytes in the Cervix. 2016

Hadijat M Makinde, and Nell Lurain, and Pincas Bitterman, and Jeff Martinson, and Jill Plants, and Alan L Landay, and Greg T Spear
Department of Microbiology and Immunology, Rush University Medical Center, Chicago, IL, USA.

OBJECTIVE Identification of the types of cells that produce IL-17 and IL-22 in the genital tract can clarify the roles that these cytokines play in responses to pathogens. METHODS We isolated and stimulated cells from cervical tissue to identify and characterize cytokine-producing cells. RESULTS Upon stimulation of CD3+ CD4+ endocervical cells, 1.6, 3.4, and 1.5% were induced to produce IL-22, IL-17, and both cytokines, respectively. Stimulation of CD3+ CD4+ ectocervical cells resulted in 3.3% IL-22+, 5.5% IL-17(+) and 2.6% IL-22(+) IL17+ cells. CD45+ CD3- cells had relatively high endogenous levels of cytokine expression that did not increase upon stimulation. Innate lymphoid cells (ILCs) made up 5.7-8% of CD45+ cervical cells and stimulation caused increases in IL-17 and IL-22. CONCLUSIONS These studies show that the majority of the CD45+ leukocytes that can be induced to produce IL-22 and IL-17 in cervix are CD3+ CD4+, but ILCs are also present and can make both cytokines.

UI MeSH Term Description Entries
D007113 Immunity, Innate The capacity of a normal organism to remain unaffected by microorganisms and their toxins. It results from the presence of naturally occurring ANTI-INFECTIVE AGENTS, constitutional factors such as BODY TEMPERATURE and immediate acting immune cells such as NATURAL KILLER CELLS. Immunity, Native,Immunity, Natural,Immunity, Non-Specific,Resistance, Natural,Innate Immune Response,Innate Immunity,Immune Response, Innate,Immune Responses, Innate,Immunity, Non Specific,Innate Immune Responses,Native Immunity,Natural Immunity,Natural Resistance,Non-Specific Immunity
D007114 Immunization Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow). Immunologic Stimulation,Immunostimulation,Sensitization, Immunologic,Variolation,Immunologic Sensitization,Immunological Stimulation,Sensitization, Immunological,Stimulation, Immunologic,Immunizations,Immunological Sensitization,Immunological Sensitizations,Immunological Stimulations,Sensitizations, Immunological,Stimulation, Immunological,Stimulations, Immunological,Variolations
D007378 Interleukins Soluble factors which stimulate growth-related activities of leukocytes as well as other cell types. They enhance cell proliferation and differentiation, DNA synthesis, secretion of other biologically active molecules and responses to immune and inflammatory stimuli. Interleukin
D007962 Leukocytes White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES). Blood Cells, White,Blood Corpuscles, White,White Blood Cells,White Blood Corpuscles,Blood Cell, White,Blood Corpuscle, White,Corpuscle, White Blood,Corpuscles, White Blood,Leukocyte,White Blood Cell,White Blood Corpuscle
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002469 Cell Separation Techniques for separating distinct populations of cells. Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002584 Cervix Uteri The neck portion of the UTERUS between the lower isthmus and the VAGINA forming the cervical canal. Cervical Canal of the Uterus,Cervical Canal, Uterine,Ectocervix,Endocervical Canal,Endocervix,External Os Cervix,External Os of the Cervix,Uterine Cervical Canal,Cervix,Cervixes,Uterine Cervix,Canal, Endocervical,Canal, Uterine Cervical,Cervix, External Os,Cervix, Uterine,Endocervical Canals,Uterine Cervical Canals
D005260 Female Females
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell

Related Publications

Hadijat M Makinde, and Nell Lurain, and Pincas Bitterman, and Jeff Martinson, and Jill Plants, and Alan L Landay, and Greg T Spear
February 2012, Clinical and experimental immunology,
Hadijat M Makinde, and Nell Lurain, and Pincas Bitterman, and Jeff Martinson, and Jill Plants, and Alan L Landay, and Greg T Spear
December 2010, Current opinion in immunology,
Hadijat M Makinde, and Nell Lurain, and Pincas Bitterman, and Jeff Martinson, and Jill Plants, and Alan L Landay, and Greg T Spear
September 2010, Trends in immunology,
Hadijat M Makinde, and Nell Lurain, and Pincas Bitterman, and Jeff Martinson, and Jill Plants, and Alan L Landay, and Greg T Spear
January 2019, Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research,
Hadijat M Makinde, and Nell Lurain, and Pincas Bitterman, and Jeff Martinson, and Jill Plants, and Alan L Landay, and Greg T Spear
October 2014, Actas dermo-sifiliograficas,
Hadijat M Makinde, and Nell Lurain, and Pincas Bitterman, and Jeff Martinson, and Jill Plants, and Alan L Landay, and Greg T Spear
January 2017, Allergologie select,
Hadijat M Makinde, and Nell Lurain, and Pincas Bitterman, and Jeff Martinson, and Jill Plants, and Alan L Landay, and Greg T Spear
April 2017, European journal of immunology,
Hadijat M Makinde, and Nell Lurain, and Pincas Bitterman, and Jeff Martinson, and Jill Plants, and Alan L Landay, and Greg T Spear
December 2016, Pathogens and disease,
Hadijat M Makinde, and Nell Lurain, and Pincas Bitterman, and Jeff Martinson, and Jill Plants, and Alan L Landay, and Greg T Spear
January 2013, Clinical & developmental immunology,
Hadijat M Makinde, and Nell Lurain, and Pincas Bitterman, and Jeff Martinson, and Jill Plants, and Alan L Landay, and Greg T Spear
October 2009, European journal of immunology,
Copied contents to your clipboard!