BIOMAT Database Logo BIOMAT Database Logo

Phosphatidylglycerol in amniotic fluid. Comparison of an "ultrasensitive" immunologic assay with TLC and enzymatic assay. 1989

A B Eisenbrey, and E Epstein, and B Zak, and R J McEnroe, and J D Artiss, and F L Kiechle
Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, Michigan 48072.

Phosphatidylglycerol (PG) in amniotic fluid is recognized as a good indicator of fetal lung maturity and is unaffected by moderate amounts of blood or meconium contamination. A rapid immunologic agglutination assay, Ultrasensitive AmnioStat-FLM (FLM), was compared with two-dimensional thin-layer chromatography (TLC) and an enzymic, colorimetric procedure (E-PG). Eighty amniotic fluid specimens were analyzed. FLM results were reported as high (H), intermediate (I), or low positive (L). TLC was compared with FLM:H (n = 27), mean 0.14 (fraction of total phospholipids); I (n = 7), mean 0.11; L (n = 9), mean 0.03; negative results had no detectable PG by TLC. In 33 cases E-PG was compared with FLM:H (n = 9), mean 7.0 mumol/L; I (n = 5), mean 8.1 mumol/L; L (n = 3), mean 3.0 mumol/L; negative (n = 16), mean 3.2 mumol/L. Records were reviewed in 70 cases. Thirty cases were excluded: sample to delivery time was greater than 72 hours; steroids were given or sepsis was documented. Fetal lung immaturity was clinically present in six cases: respiratory distress syndrome in three cases and transient tachypnea of the newborn (TTN) in three cases. One false positive result was identified (TTN, FLM:H). FLM sensitivity for fetal lung maturity was 85.3%, specificity was 83.3%, and the positive predictive value for fetal lung maturity was 96.7%. FLM is a fast, reliable indicator of fetal lung maturity.

UI MeSH Term Description Entries
D008168 Lung Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood. Lungs
D009994 Osmolar Concentration The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent. Ionic Strength,Osmolality,Osmolarity,Concentration, Osmolar,Concentrations, Osmolar,Ionic Strengths,Osmolalities,Osmolar Concentrations,Osmolarities,Strength, Ionic,Strengths, Ionic
D010715 Phosphatidylglycerols A nitrogen-free class of lipids present in animal and particularly plant tissues and composed of one mole of glycerol and 1 or 2 moles of phosphatidic acid. Members of this group differ from one another in the nature of the fatty acids released on hydrolysis. Glycerol Phosphoglycerides,Monophosphatidylglycerols,Phosphatidylglycerol,Phosphatidyl Glycerol,Glycerol, Phosphatidyl,Phosphoglycerides, Glycerol
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D011933 Reagent Kits, Diagnostic Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use. Diagnostic Reagent Kits,Diagnostic Reagents and Test Kits,Diagnostic Test Kits,In Vitro Diagnostic Device,In Vitro Diagnostic Devices,In Vitro Diagnostic Medical Device,In Vitro Diagnostic Medical Devices,Kits, Diagnostic Reagent,Diagnostic Reagent Kit,Diagnostic Test Kit,Kit, Diagnostic Reagent,Kit, Diagnostic Test,Kits, Diagnostic Test,Reagent Kit, Diagnostic,Test Kit, Diagnostic,Test Kits, Diagnostic
D002855 Chromatography, Thin Layer Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Thin-Layer,Thin Layer Chromatography,Chromatographies, Thin Layer,Chromatographies, Thin-Layer,Thin Layer Chromatographies,Thin-Layer Chromatographies,Thin-Layer Chromatography
D003124 Colorimetry Any technique by which an unknown color is evaluated in terms of standard colors. The technique may be visual, photoelectric, or indirect by means of spectrophotometry. It is used in chemistry and physics. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D004798 Enzymes Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified. Biocatalyst,Enzyme,Biocatalysts
D005260 Female Females
D005314 Embryonic and Fetal Development Morphological and physiological development of EMBRYOS or FETUSES. Embryo and Fetal Development,Prenatal Programming,Programming, Prenatal

Related Publications

A B Eisenbrey, and E Epstein, and B Zak, and R J McEnroe, and J D Artiss, and F L Kiechle
Combined enzymatic assay of phosphatidylglycerol and phosphatidylcholine in amniotic fluid.
February 1988, Clinica chimica acta; international journal of clinical chemistry,
A B Eisenbrey, and E Epstein, and B Zak, and R J McEnroe, and J D Artiss, and F L Kiechle
Quantitative determination of phosphatidylglycerol in amniotic fluid by enzymatic assay.
October 1985, Clinica chimica acta; international journal of clinical chemistry,
A B Eisenbrey, and E Epstein, and B Zak, and R J McEnroe, and J D Artiss, and F L Kiechle
Interference by endogenous glycerol in an enzymatic assay of phosphatidylglycerol in amniotic fluid.
March 1988, Clinical chemistry,
A B Eisenbrey, and E Epstein, and B Zak, and R J McEnroe, and J D Artiss, and F L Kiechle
Enzymatic measurement of phosphatidylglycerol in amniotic fluid.
April 1994, Clinical chemistry,
A B Eisenbrey, and E Epstein, and B Zak, and R J McEnroe, and J D Artiss, and F L Kiechle
[Enzymatic colorimetric determination of phosphatidylglycerol in amniotic fluid].
March 1990, Nihon Sanka Fujinka Gakkai zasshi,
A B Eisenbrey, and E Epstein, and B Zak, and R J McEnroe, and J D Artiss, and F L Kiechle
[Phosphatidylglycerol in amniotic fluid. Value of rapid determination using an immunologic technic].
January 1985, Journal de gynecologie, obstetrique et biologie de la reproduction,
A B Eisenbrey, and E Epstein, and B Zak, and R J McEnroe, and J D Artiss, and F L Kiechle
Automated enzymatic measurement of lecithin, sphingomyelin, and phosphatidylglycerol in amniotic fluid.
January 1987, Clinical chemistry,
A B Eisenbrey, and E Epstein, and B Zak, and R J McEnroe, and J D Artiss, and F L Kiechle
A cost-effective modification of an immunologic agglutination test for amniotic fluid phosphatidylglycerol.
July 1984, Clinical chemistry,
A B Eisenbrey, and E Epstein, and B Zak, and R J McEnroe, and J D Artiss, and F L Kiechle
Phosphatidylglycerol in amniotic fluid.
March 1986, Clinical chemistry,
A B Eisenbrey, and E Epstein, and B Zak, and R J McEnroe, and J D Artiss, and F L Kiechle
[A sensitive enzymatic method for the determination of phosphatidylglycerol in amniotic fluid].
April 1989, Nihon Sanka Fujinka Gakkai zasshi,
Copied contents to your clipboard!