-transparent.png){kind=logo}
A highly specific and sensitive radioimmunoassay has been established for measurement of human endothelin (hET) in human plasma. After extraction of plasma with an octyl-silica column, this assay allowed for detection of immunoreactive (IR) hET as low as 0.2 fmol/ml. In 16 healthy subjects, the mean concentration of plasma IR-hET was 0.6 fmol/ml. Reverse-phase HPLC coupled with radioimmunoassay revealed two major IR-hET components, one corresponding to authentic hET(1-21) and another with more hydrophilicity than hET(1-21). These data indicate that ET is a circulating vasoconstrictor hormone in man.
Related Publications
K Ando, and
Y Hirata, and
M Shichiri, and
T Emori, and
F Marumo
February 1990,
FEBS letters,
K Ando, and
Y Hirata, and
M Shichiri, and
T Emori, and
F Marumo
May 1989,
Lancet (London, England),
K Ando, and
Y Hirata, and
M Shichiri, and
T Emori, and
F Marumo
January 1991,
Journal of cardiovascular pharmacology,
K Ando, and
Y Hirata, and
M Shichiri, and
T Emori, and
F Marumo
March 1991,
The Journal of clinical endocrinology and metabolism,
K Ando, and
Y Hirata, and
M Shichiri, and
T Emori, and
F Marumo
January 1991,
Peptides,
K Ando, and
Y Hirata, and
M Shichiri, and
T Emori, and
F Marumo
June 1990,
The American journal of medicine,
K Ando, and
Y Hirata, and
M Shichiri, and
T Emori, and
F Marumo
January 1991,
Journal of cardiovascular pharmacology,
K Ando, and
Y Hirata, and
M Shichiri, and
T Emori, and
F Marumo
May 1991,
Annals of clinical biochemistry,
K Ando, and
Y Hirata, and
M Shichiri, and
T Emori, and
F Marumo
January 1998,
Life sciences,
K Ando, and
Y Hirata, and
M Shichiri, and
T Emori, and
F Marumo
November 1991,
Nihon Geka Gakkai zasshi,
