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A cloned repetitive DNA sequence (rep20) was evaluated as a diagnostic probe specific for Plasmodium falciparum sporozoites using experimentally infected mosquitoes squashed directly on nylon filters. Head/thorax portions of mosquitoes, killed 14-16 days after ingesting P. falciparum-infected blood, gave positive signals when examined for the presence of P. falciparum sporozoite DNA by hybridisation. This correlated with the number of oocysts found in a sample of the same batch of mosquitoes examined by dissection. No positive signals were obtained with 50 Plasmodium berghei-infected mosquitoes probed with the rep20 sequence. The results indicate that a probe containing rep20 may be useful in the rapid and specific incrimination of vectors carrying P. falciparum sporozoites. The value of repetitive DNA in the diagnosis of malaria is discussed.
C J Delves, and
M Goman, and
R G Ridley, and
H Matile, and
T H Lensen, and
T Ponnudurai, and
J G Scaife
January 1988,
Transactions of the Royal Society of Tropical Medicine and Hygiene,
C J Delves, and
M Goman, and
R G Ridley, and
H Matile, and
T H Lensen, and
T Ponnudurai, and
J G Scaife
September 1985,
The American journal of tropical medicine and hygiene,
C J Delves, and
M Goman, and
R G Ridley, and
H Matile, and
T H Lensen, and
T Ponnudurai, and
J G Scaife
January 1989,
Transactions of the Royal Society of Tropical Medicine and Hygiene,
C J Delves, and
M Goman, and
R G Ridley, and
H Matile, and
T H Lensen, and
T Ponnudurai, and
J G Scaife
June 1988,
Molecular and biochemical parasitology,
C J Delves, and
M Goman, and
R G Ridley, and
H Matile, and
T H Lensen, and
T Ponnudurai, and
J G Scaife
September 1984,
The American journal of tropical medicine and hygiene,
C J Delves, and
M Goman, and
R G Ridley, and
H Matile, and
T H Lensen, and
T Ponnudurai, and
J G Scaife
June 1995,
Journal of the American Mosquito Control Association,
C J Delves, and
M Goman, and
R G Ridley, and
H Matile, and
T H Lensen, and
T Ponnudurai, and
J G Scaife
January 1997,
Acta tropica,
C J Delves, and
M Goman, and
R G Ridley, and
H Matile, and
T H Lensen, and
T Ponnudurai, and
J G Scaife
March 1984,
Lancet (London, England),
C J Delves, and
M Goman, and
R G Ridley, and
H Matile, and
T H Lensen, and
T Ponnudurai, and
J G Scaife
January 1987,
Molecular and biochemical parasitology,
C J Delves, and
M Goman, and
R G Ridley, and
H Matile, and
T H Lensen, and
T Ponnudurai, and
J G Scaife
January 1989,
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases,
