-transparent.png){kind=logo}
The direct measurement of mass of inositol trisphosphate from biologic samples is described. Separation of inositol monophosphate, bisphosphate, trisphosphate, and inositol tetrakisphosphate was achieved using anion exchange chromatography with a sodium sulfate gradient. In addition, separation of the isomers of each inositol phosphate was performed using HPLC procedures. The individual inositol phosphate fractions were subsequently dephosphorylated and desalted. The myo-inositol from each fraction was then derivatized to the hexatrimethylsilyl derivative and the myo-inositol derivatives were quantified by a novel gas chromatographic analysis using the hexatrimethylsilyl derivative of chiro-inositol as an internal concentration reference. This method is a reproducible and relatively rapid procedure for the direct quantification of inositol phosphate mass which overcomes many of the problems associated with the use of radiolabeled precursors. The method is a significant improvement over existing procedures for the quantitative determination of the mass of inositol phosphate by virtue of improved recovery, sensitivity, and technical simplicity. The applicability of this method is illustrated by the quantitative determination of inositol trisphosphate in response to norepinephrine stimulation of adult canine myocytes and cerebral cortical brain slices and by measurement of the isomers of inositol trisphosphate in isolated myocytes.
G P Heathers, and
T Juehne, and
L J Rubin, and
P B Corr, and
A S Evers
April 1988,
The Analyst,
G P Heathers, and
T Juehne, and
L J Rubin, and
P B Corr, and
A S Evers
January 1988,
Analytical biochemistry,
G P Heathers, and
T Juehne, and
L J Rubin, and
P B Corr, and
A S Evers
August 1970,
Clinical chemistry,
G P Heathers, and
T Juehne, and
L J Rubin, and
P B Corr, and
A S Evers
February 1962,
Archives of biochemistry and biophysics,
G P Heathers, and
T Juehne, and
L J Rubin, and
P B Corr, and
A S Evers
July 1985,
Analytical biochemistry,
G P Heathers, and
T Juehne, and
L J Rubin, and
P B Corr, and
A S Evers
November 1990,
Journal of chromatography,
G P Heathers, and
T Juehne, and
L J Rubin, and
P B Corr, and
A S Evers
July 1997,
Molecular and cellular biochemistry,
G P Heathers, and
T Juehne, and
L J Rubin, and
P B Corr, and
A S Evers
January 2021,
Methods in molecular biology (Clifton, N.J.),
G P Heathers, and
T Juehne, and
L J Rubin, and
P B Corr, and
A S Evers
February 1988,
Biochemical and biophysical research communications,
G P Heathers, and
T Juehne, and
L J Rubin, and
P B Corr, and
A S Evers
August 2012,
Sheng wu gong cheng xue bao = Chinese journal of biotechnology,
