BIOMAT Database Logo BIOMAT Database Logo

miR-296-5p suppresses cell viability by directly targeting PLK1 in non-small cell lung cancer. 2016

Chun Xu, and Sen Li, and Tengfei Chen, and Haibo Hu, and Cheng Ding, and Zhenlei Xu, and Jun Chen, and Zeyi Liu, and Zhe Lei, and Hong-Tao Zhang, and Chang Li, and Jun Zhao
Department of Thoracic and Cardiovascular Surgery, the First Affiliated Hospital of Soochow University, Medical College of Soochow University, Suzhou, Jiangsu 215006, P.R. China.

Polo-like kinase 1 (PLK1), a critical kinase for mitotic progression, is overexpressed in a wide range of cancers. MicroRNAs (miRNAs) are a class of small non-coding RNA molecules and proposed to play important roles in the regulation of tumor progression and invasion. However, the relationship between PLK1 and miRNAs have remained unclear. In the present study, the association between PLK1 and miR-296-5p was investigated. The upregulation of PLK1 mRNA expression levels combined with the downregulation of miR-296-5p levels were detected in both non-small cell lung cancer (NSCLC) tissues and cell lines. Functional studies showed that knockdown of PLK1 by siRNA inhibited NSCLC cells proliferation. Impressively, overexpression of miR-296-5p showed the same phenocopy as the effect of PLK1 knockdown in NSCLC cells, indicating that PLK1 was a major target of miR-296-5p. Furthermore, using western blot analysis and luciferase reporter assay, PLK1 protein expression was proved to be regulated by miR-296-5p through binding to the putative binding sites in its 3'-untranslated region (3'-UTR). Taken together, the present study indicated that miR-296-5p regulated PLK1 expression and could function as a tumor suppressor in NSCLC progression, which provides a potential target for gene therapy of NSCLC.

UI MeSH Term Description Entries
D008175 Lung Neoplasms Tumors or cancer of the LUNG. Cancer of Lung,Lung Cancer,Pulmonary Cancer,Pulmonary Neoplasms,Cancer of the Lung,Neoplasms, Lung,Neoplasms, Pulmonary,Cancer, Lung,Cancer, Pulmonary,Cancers, Lung,Cancers, Pulmonary,Lung Cancers,Lung Neoplasm,Neoplasm, Lung,Neoplasm, Pulmonary,Pulmonary Cancers,Pulmonary Neoplasm
D011518 Proto-Oncogene Proteins Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity. Cellular Proto-Oncogene Proteins,c-onc Proteins,Proto Oncogene Proteins, Cellular,Proto-Oncogene Products, Cellular,Cellular Proto Oncogene Proteins,Cellular Proto-Oncogene Products,Proto Oncogene Products, Cellular,Proto Oncogene Proteins,Proto-Oncogene Proteins, Cellular,c onc Proteins
D002289 Carcinoma, Non-Small-Cell Lung A heterogeneous aggregate of at least three distinct histological types of lung cancer, including SQUAMOUS CELL CARCINOMA; ADENOCARCINOMA; and LARGE CELL CARCINOMA. They are dealt with collectively because of their shared treatment strategy. Carcinoma, Non-Small Cell Lung,Non-Small Cell Lung Cancer,Non-Small Cell Lung Carcinoma,Non-Small-Cell Lung Carcinoma,Nonsmall Cell Lung Cancer,Carcinoma, Non Small Cell Lung,Carcinomas, Non-Small-Cell Lung,Lung Carcinoma, Non-Small-Cell,Lung Carcinomas, Non-Small-Cell,Non Small Cell Lung Carcinoma,Non-Small-Cell Lung Carcinomas
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000097869 Polo-Like Kinase 1 Plays a crucial role in the regulation of proliferative activity of normal and malignant cells. Proposed as a new target for antineoplastic treatment strategies. Plk1 protein,STPK13 protein,Polo Like Kinase 1
D015972 Gene Expression Regulation, Neoplastic Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue. Neoplastic Gene Expression Regulation,Regulation of Gene Expression, Neoplastic,Regulation, Gene Expression, Neoplastic
D017346 Protein Serine-Threonine Kinases A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors. Protein-Serine-Threonine Kinases,Serine-Threonine Protein Kinase,Serine-Threonine Protein Kinases,Protein-Serine Kinase,Protein-Serine-Threonine Kinase,Protein-Threonine Kinase,Serine Kinase,Serine-Threonine Kinase,Serine-Threonine Kinases,Threonine Kinase,Kinase, Protein-Serine,Kinase, Protein-Serine-Threonine,Kinase, Protein-Threonine,Kinase, Serine-Threonine,Kinases, Protein Serine-Threonine,Kinases, Protein-Serine-Threonine,Kinases, Serine-Threonine,Protein Kinase, Serine-Threonine,Protein Kinases, Serine-Threonine,Protein Serine Kinase,Protein Serine Threonine Kinase,Protein Serine Threonine Kinases,Protein Threonine Kinase,Serine Threonine Kinase,Serine Threonine Kinases,Serine Threonine Protein Kinase,Serine Threonine Protein Kinases
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D049109 Cell Proliferation All of the processes involved in increasing CELL NUMBER including CELL DIVISION. Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular
D018797 Cell Cycle Proteins Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS. Cell Division Cycle Proteins,Cell-Cycle Regulatory Proteins,cdc Proteins,Cell Cycle Regulatory Proteins

Related Publications

Chun Xu, and Sen Li, and Tengfei Chen, and Haibo Hu, and Cheng Ding, and Zhenlei Xu, and Jun Chen, and Zeyi Liu, and Zhe Lei, and Hong-Tao Zhang, and Chang Li, and Jun Zhao
MiR-296-5p suppresses papillary thyroid carcinoma cell growth via targeting PLK1.
March 2019, European review for medical and pharmacological sciences,
Chun Xu, and Sen Li, and Tengfei Chen, and Haibo Hu, and Cheng Ding, and Zhenlei Xu, and Jun Chen, and Zeyi Liu, and Zhe Lei, and Hong-Tao Zhang, and Chang Li, and Jun Zhao
MiR-142-5p Suppresses Tumorigenesis by Targeting PIK3CA in Non-Small Cell Lung Cancer.
January 2017, Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology,
Chun Xu, and Sen Li, and Tengfei Chen, and Haibo Hu, and Cheng Ding, and Zhenlei Xu, and Jun Chen, and Zeyi Liu, and Zhe Lei, and Hong-Tao Zhang, and Chang Li, and Jun Zhao
MiR-199a-5p suppresses non-small cell lung cancer via targeting MAP3K11.
January 2019, Journal of Cancer,
Chun Xu, and Sen Li, and Tengfei Chen, and Haibo Hu, and Cheng Ding, and Zhenlei Xu, and Jun Chen, and Zeyi Liu, and Zhe Lei, and Hong-Tao Zhang, and Chang Li, and Jun Zhao
MiR-7-5p suppresses tumor metastasis of non-small cell lung cancer by targeting NOVA2.
January 2019, Cellular & molecular biology letters,
Chun Xu, and Sen Li, and Tengfei Chen, and Haibo Hu, and Cheng Ding, and Zhenlei Xu, and Jun Chen, and Zeyi Liu, and Zhe Lei, and Hong-Tao Zhang, and Chang Li, and Jun Zhao
MicroRNA-296-5p (miR-296-5p) functions as a tumor suppressor in prostate cancer by directly targeting Pin1.
September 2014, Biochimica et biophysica acta,
Chun Xu, and Sen Li, and Tengfei Chen, and Haibo Hu, and Cheng Ding, and Zhenlei Xu, and Jun Chen, and Zeyi Liu, and Zhe Lei, and Hong-Tao Zhang, and Chang Li, and Jun Zhao
miR-744-5p Inhibits Non-Small Cell Lung Cancer Proliferation and Invasion by Directly Targeting PAX2.
January 2019, Technology in cancer research & treatment,
Chun Xu, and Sen Li, and Tengfei Chen, and Haibo Hu, and Cheng Ding, and Zhenlei Xu, and Jun Chen, and Zeyi Liu, and Zhe Lei, and Hong-Tao Zhang, and Chang Li, and Jun Zhao
MiR-196b-5p activates NF-κB signaling in non-small cell lung cancer by directly targeting NFKBIA.
November 2023, Translational oncology,
Chun Xu, and Sen Li, and Tengfei Chen, and Haibo Hu, and Cheng Ding, and Zhenlei Xu, and Jun Chen, and Zeyi Liu, and Zhe Lei, and Hong-Tao Zhang, and Chang Li, and Jun Zhao
Non-small cell lung cancer: miR-30d suppresses tumor invasion and migration by directly targeting NFIB.
December 2017, Biotechnology letters,
Chun Xu, and Sen Li, and Tengfei Chen, and Haibo Hu, and Cheng Ding, and Zhenlei Xu, and Jun Chen, and Zeyi Liu, and Zhe Lei, and Hong-Tao Zhang, and Chang Li, and Jun Zhao
miR-512-5p suppresses the progression of non-small cell lung cancer by targeting β-catenin.
January 2020, Oncology letters,
Chun Xu, and Sen Li, and Tengfei Chen, and Haibo Hu, and Cheng Ding, and Zhenlei Xu, and Jun Chen, and Zeyi Liu, and Zhe Lei, and Hong-Tao Zhang, and Chang Li, and Jun Zhao
MicroRNA-107-5p suppresses non-small cell lung cancer by directly targeting oncogene epidermal growth factor receptor.
August 2017, Oncotarget,
Copied contents to your clipboard!