A nonsialylated high-molecular-weight glycoprotein defined by a monoclonal antibody to adenocarcinoma of the uterine endometrium. 1989

M Inoue, and T Sasagawa, and C Shimizu, and H Shimizu, and J Saito, and G Ueda, and O Tanizawa
Department of Obstetrics and Gynecology, Osaka University School of Medicine, Japan.

A murine monoclonal antibody, MCA-97, was prepared against a human endometrial adenocarcinoma cell line. In a cellular enzyme-linked immunospecific assay, the MCA-97 antibody reacted with all adenocarcinoma cell lines tested, including four endometrial adenocarcinoma lines. The antigen defined by MCA-97 was estimated to be a nonsialylated high-molecular-weight glycoprotein containing galactose and N-acetylglucosamine in its determinant. In immunoperoxidase staining of formalin-fixed, paraffin-embedded sections, MCA-97 reacted with most endometrial adenocarcinomas and ovarian endometrioid-type adenocarcinomas, and also reacted with normal glandular epithelium of the female genital and gastrointestinal tracts. However, it was mostly unreactive with squamous cell carcinoma and ovarian serous adenocarcinoma tissues. By the reversed passive hemagglutination method, the antigen defined by MCA-97 was detected in the sera of one-third of patients with endometrial adenocarcinomas, and in half of those with ovarian endometrioid adenocarcinomas. The antigen was not demonstrable in the sera of most normal female volunteers. Thus, MCA-97 monoclonal antibody has potential clinical applications in diagnostic serology and pathology.

UI MeSH Term Description Entries
D007124 Immunoenzyme Techniques Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens. Antibody Enzyme Technique, Unlabeled,Enzyme Immunoassay,Enzyme-Labeled Antibody Technique,Immunoassay, Enzyme,Immunoperoxidase Techniques,Peroxidase-Antiperoxidase Complex Technique,Peroxidase-Labeled Antibody Technique,Antibody Enzyme Technic, Unlabeled,Enzyme-Labeled Antibody Technic,Immunoenzyme Technics,Immunoperoxidase Technics,Peroxidase-Antiperoxidase Complex Technic,Peroxidase-Labeled Antibody Technic,Antibody Technic, Enzyme-Labeled,Antibody Technic, Peroxidase-Labeled,Antibody Technics, Enzyme-Labeled,Antibody Technics, Peroxidase-Labeled,Antibody Technique, Enzyme-Labeled,Antibody Technique, Peroxidase-Labeled,Antibody Techniques, Enzyme-Labeled,Antibody Techniques, Peroxidase-Labeled,Enzyme Immunoassays,Enzyme Labeled Antibody Technic,Enzyme Labeled Antibody Technique,Enzyme-Labeled Antibody Technics,Enzyme-Labeled Antibody Techniques,Immunoassays, Enzyme,Immunoenzyme Technic,Immunoenzyme Technique,Immunoperoxidase Technic,Immunoperoxidase Technique,Peroxidase Antiperoxidase Complex Technic,Peroxidase Antiperoxidase Complex Technique,Peroxidase Labeled Antibody Technic,Peroxidase Labeled Antibody Technique,Peroxidase-Antiperoxidase Complex Technics,Peroxidase-Antiperoxidase Complex Techniques,Peroxidase-Labeled Antibody Technics,Peroxidase-Labeled Antibody Techniques,Technic, Enzyme-Labeled Antibody,Technic, Immunoenzyme,Technic, Immunoperoxidase,Technic, Peroxidase-Antiperoxidase Complex,Technic, Peroxidase-Labeled Antibody,Technics, Enzyme-Labeled Antibody,Technics, Immunoenzyme,Technics, Immunoperoxidase,Technics, Peroxidase-Antiperoxidase Complex,Technics, Peroxidase-Labeled Antibody,Technique, Enzyme-Labeled Antibody,Technique, Immunoenzyme,Technique, Immunoperoxidase,Technique, Peroxidase-Antiperoxidase Complex,Technique, Peroxidase-Labeled Antibody,Techniques, Enzyme-Labeled Antibody,Techniques, Immunoenzyme,Techniques, Immunoperoxidase,Techniques, Peroxidase-Antiperoxidase Complex,Techniques, Peroxidase-Labeled Antibody
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D009369 Neoplasms New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms. Benign Neoplasm,Cancer,Malignant Neoplasm,Tumor,Tumors,Benign Neoplasms,Malignancy,Malignant Neoplasms,Neoplasia,Neoplasm,Neoplasms, Benign,Cancers,Malignancies,Neoplasias,Neoplasm, Benign,Neoplasm, Malignant,Neoplasms, Malignant
D010051 Ovarian Neoplasms Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS. Cancer of Ovary,Ovarian Cancer,Cancer of the Ovary,Neoplasms, Ovarian,Ovary Cancer,Ovary Neoplasms,Cancer, Ovarian,Cancer, Ovary,Cancers, Ovarian,Cancers, Ovary,Neoplasm, Ovarian,Neoplasm, Ovary,Neoplasms, Ovary,Ovarian Cancers,Ovarian Neoplasm,Ovary Cancers,Ovary Neoplasm
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260 Female Females
D006023 Glycoproteins Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins. C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D006386 Hemagglutination Tests Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed) Hemagglutination Test,Test, Hemagglutination,Tests, Hemagglutination
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000230 Adenocarcinoma A malignant epithelial tumor with a glandular organization. Adenocarcinoma, Basal Cell,Adenocarcinoma, Granular Cell,Adenocarcinoma, Oxyphilic,Adenocarcinoma, Tubular,Adenoma, Malignant,Carcinoma, Cribriform,Carcinoma, Granular Cell,Carcinoma, Tubular,Adenocarcinomas,Adenocarcinomas, Basal Cell,Adenocarcinomas, Granular Cell,Adenocarcinomas, Oxyphilic,Adenocarcinomas, Tubular,Adenomas, Malignant,Basal Cell Adenocarcinoma,Basal Cell Adenocarcinomas,Carcinomas, Cribriform,Carcinomas, Granular Cell,Carcinomas, Tubular,Cribriform Carcinoma,Cribriform Carcinomas,Granular Cell Adenocarcinoma,Granular Cell Adenocarcinomas,Granular Cell Carcinoma,Granular Cell Carcinomas,Malignant Adenoma,Malignant Adenomas,Oxyphilic Adenocarcinoma,Oxyphilic Adenocarcinomas,Tubular Adenocarcinoma,Tubular Adenocarcinomas,Tubular Carcinoma,Tubular Carcinomas

Related Publications

M Inoue, and T Sasagawa, and C Shimizu, and H Shimizu, and J Saito, and G Ueda, and O Tanizawa
April 1988, Hybridoma,
M Inoue, and T Sasagawa, and C Shimizu, and H Shimizu, and J Saito, and G Ueda, and O Tanizawa
March 1988, British journal of cancer,
M Inoue, and T Sasagawa, and C Shimizu, and H Shimizu, and J Saito, and G Ueda, and O Tanizawa
January 1984, Journal of neuroimmunology,
M Inoue, and T Sasagawa, and C Shimizu, and H Shimizu, and J Saito, and G Ueda, and O Tanizawa
February 1985, Cancer research,
M Inoue, and T Sasagawa, and C Shimizu, and H Shimizu, and J Saito, and G Ueda, and O Tanizawa
April 1990, Laboratory investigation; a journal of technical methods and pathology,
M Inoue, and T Sasagawa, and C Shimizu, and H Shimizu, and J Saito, and G Ueda, and O Tanizawa
April 1993, Cancer,
M Inoue, and T Sasagawa, and C Shimizu, and H Shimizu, and J Saito, and G Ueda, and O Tanizawa
April 1985, Cancer research,
M Inoue, and T Sasagawa, and C Shimizu, and H Shimizu, and J Saito, and G Ueda, and O Tanizawa
December 1985, Experientia,
M Inoue, and T Sasagawa, and C Shimizu, and H Shimizu, and J Saito, and G Ueda, and O Tanizawa
June 1984, Gan,
M Inoue, and T Sasagawa, and C Shimizu, and H Shimizu, and J Saito, and G Ueda, and O Tanizawa
June 1985, The Journal of biological chemistry,
Copied contents to your clipboard!