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Characterization of siRNAs derived from cucumber green mottle mosaic virus in infected cucumber plants. 2016
Yongqiang Li, and
Congliang Deng, and
Qiaoxia Shang, and
Xiaoli Zhao, and
Xingliang Liu, and
Qi Zhou
College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China. lyq047@163.com.
Virus-derived small interfering RNAs (vsiRNAs) of cucumber green mottle mosaic virus (CGMMV), a member of the genus Tobamovirus, were characterised in cucumber plants by deep sequencing. CGMMV vsiRNAs of 21-22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CGMMV-infected cucumber plants. The 5'-terminal base of vsiRNAs was biased towards C/A/U, suggesting that CGMMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were also predicted.
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