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A test system based on immunochromatography in the sandwich format and intended for express detection of Helicobacter pylori antigens has been developed. Contact of a sample with a test strip coated with immunochemical reagents triggers the movement of the liquid along the membrane components of the test strip, immunochemical interactions, and the formation of detection zones stained by gold nanoparticles. The concentration and kinetic dependences of the immunochemical interactions have been characterized. The reagent and membrane composition of the test system has been selected to provide a minimal detection limit. The detection of H. pylori cell wall antigens at concentrations as low as 0.3 μg/mL in aqueous solution and a suspension of a clinical sample of feces has been demonstrated; the assay duration was 10 minutes. Staining enhancement by the addition of silver salts allowed for a further reduction of the detection limit to 0.03 μg/mL. The developed test system can be used for field diagnostics.
N A Byzova, and
A V Zherdev, and
P G Sveshnikov, and
E G Sadykhov, and
B B Dzantiev
April 2018,
Clinics and research in hepatology and gastroenterology,
N A Byzova, and
A V Zherdev, and
P G Sveshnikov, and
E G Sadykhov, and
B B Dzantiev
December 2015,
Clinical biochemistry,
N A Byzova, and
A V Zherdev, and
P G Sveshnikov, and
E G Sadykhov, and
B B Dzantiev
July 1997,
The American journal of gastroenterology,
N A Byzova, and
A V Zherdev, and
P G Sveshnikov, and
E G Sadykhov, and
B B Dzantiev
January 2015,
Journal of the Medical Association of Thailand = Chotmaihet thangphaet,
N A Byzova, and
A V Zherdev, and
P G Sveshnikov, and
E G Sadykhov, and
B B Dzantiev
April 2020,
Journal of chromatographic science,
N A Byzova, and
A V Zherdev, and
P G Sveshnikov, and
E G Sadykhov, and
B B Dzantiev
January 2002,
Acta gastro-enterologica Belgica,
N A Byzova, and
A V Zherdev, and
P G Sveshnikov, and
E G Sadykhov, and
B B Dzantiev
March 1993,
Ceskoslovenska epidemiologie, mikrobiologie, imunologie,
N A Byzova, and
A V Zherdev, and
P G Sveshnikov, and
E G Sadykhov, and
B B Dzantiev
January 2013,
BioMed research international,
N A Byzova, and
A V Zherdev, and
P G Sveshnikov, and
E G Sadykhov, and
B B Dzantiev
November 1994,
European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP),
N A Byzova, and
A V Zherdev, and
P G Sveshnikov, and
E G Sadykhov, and
B B Dzantiev
February 1998,
Clinical chemistry,
