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A method has been developed to detect chemically induced aneuploidy in interphase nuclei by means of in situ hybridization with chromosome-specific DNA probes. Lymphocyte cultures were treated with two known aneuploidy inducers, Benomyl and Griseofulvin. Two DNA fragments, QP23 and Y97, homologous to repetitive sequences, localized in the pericentromeric region of chromosome 9 and in the centromeric region of Y chromosome respectively, were used as probes. Following autoradiography, grain clusters, revealing the presence of the target chromosomes, were scored in resting nuclei. A marked increase in the frequency of cells with supernumerary autoradiographic signals was observed with both probes at all concentrations of the test compounds. The assay procedure appeared to be reproducible, sensitive and efficient in scoring large cell samples. It may therefore provide a useful tool for preliminary screening of potential aneuploidy inducers.
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February 1990,
Mutation research,
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January 1989,
Progress in clinical and biological research,
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July 1996,
Cancer genetics and cytogenetics,
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July 1992,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
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January 1984,
Human genetics,
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February 1998,
Mayo Clinic proceedings,
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August 1997,
Mayo Clinic proceedings,
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December 1994,
Prenatal diagnosis,
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January 1993,
Cytogenetics and cell genetics,
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March 1996,
Diagnostic cytopathology,
