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Effective Synthesis of Fluorescently Labeled Morpholino Nucleoside Triphosphate Derivatives. 2016
a Institute of Chemical Biology and Fundamental Medicine SB RAS , Lavrent'ev Ave 8, Novosibirsk 630090 , Russian Federation.
Morpholino nucleoside triphosphates (A, U, G, C, T) bearing the active functional amino group tethered to morpholine residue and their fluorescently labeled derivatives were synthesized. All compounds were characterized by (1)H, (13)C, and (31)P NMR, and mass spectrometry. A possibility of using fluorescently labeled morpholino nucleoside triphosphates as chain terminators in DNA sequencing is discussed.
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