-transparent.png){kind=logo}
Microbial communities from 20 different hydrogen-producing reactors studied by 454 pyrosequencing. 2016
Claudia Etchebehere, and
Elena Castelló, and
Jorge Wenzel, and
Mélida del Pilar Anzola-Rojas, and
Liliana Borzacconi, and
Germán Buitrón, and
Lea Cabrol, and
Vivian María Carminato, and
Julian Carrillo-Reyes, and
Crhistian Cisneros-Pérez, and
Laura Fuentes, and
Iván Moreno-Andrade, and
Elías Razo-Flores, and
Gonzalo Ruiz Filippi, and
Estela Tapia-Venegas, and
Javiera Toledo-Alarcón, and
Marcelo Zaiat
Microbial Ecology Laboratory, BioGem Department, Biological Research Institute Clemente Estable, Ministery of Education, Av. Italia, 3318, Montevideo, Uruguay. cetchebehere@iibce.edu.uy.
To provide new insight into the dark fermentation process, a multi-lateral study was performed to study the microbiology of 20 different lab-scale bioreactors operated in four different countries (Brazil, Chile, Mexico, and Uruguay). Samples (29) were collected from bioreactors with different configurations, operation conditions, and performances. The microbial communities were analyzed using 16S rRNA genes 454 pyrosequencing. The results showed notably uneven communities with a high predominance of a particular genus. The phylum Firmicutes predominated in most of the samples, but the phyla Thermotogae or Proteobacteria dominated in a few samples. Genera from three physiological groups were detected: high-yield hydrogen producers (Clostridium, Kosmotoga, Enterobacter), fermenters with low-hydrogen yield (mostly from Veillonelaceae), and competitors (Lactobacillus). Inocula, reactor configurations, and substrates influence the microbial communities. This is the first joint effort that evaluates hydrogen-producing reactors and operational conditions from different countries and contributes to understand the dark fermentation process.
Related Publications
Claudia Etchebehere, and
Elena Castelló, and
Jorge Wenzel, and
Mélida del Pilar Anzola-Rojas, and
Liliana Borzacconi, and
Germán Buitrón, and
Lea Cabrol, and
Vivian María Carminato, and
Julian Carrillo-Reyes, and
Crhistian Cisneros-Pérez, and
Laura Fuentes, and
Iván Moreno-Andrade, and
Elías Razo-Flores, and
Gonzalo Ruiz Filippi, and
Estela Tapia-Venegas, and
Javiera Toledo-Alarcón, and
Marcelo Zaiat
April 2015,
Microbiological research,
Claudia Etchebehere, and
Elena Castelló, and
Jorge Wenzel, and
Mélida del Pilar Anzola-Rojas, and
Liliana Borzacconi, and
Germán Buitrón, and
Lea Cabrol, and
Vivian María Carminato, and
Julian Carrillo-Reyes, and
Crhistian Cisneros-Pérez, and
Laura Fuentes, and
Iván Moreno-Andrade, and
Elías Razo-Flores, and
Gonzalo Ruiz Filippi, and
Estela Tapia-Venegas, and
Javiera Toledo-Alarcón, and
Marcelo Zaiat
December 2010,
Molecular ecology,
Claudia Etchebehere, and
Elena Castelló, and
Jorge Wenzel, and
Mélida del Pilar Anzola-Rojas, and
Liliana Borzacconi, and
Germán Buitrón, and
Lea Cabrol, and
Vivian María Carminato, and
Julian Carrillo-Reyes, and
Crhistian Cisneros-Pérez, and
Laura Fuentes, and
Iván Moreno-Andrade, and
Elías Razo-Flores, and
Gonzalo Ruiz Filippi, and
Estela Tapia-Venegas, and
Javiera Toledo-Alarcón, and
Marcelo Zaiat
January 2016,
SpringerPlus,
Claudia Etchebehere, and
Elena Castelló, and
Jorge Wenzel, and
Mélida del Pilar Anzola-Rojas, and
Liliana Borzacconi, and
Germán Buitrón, and
Lea Cabrol, and
Vivian María Carminato, and
Julian Carrillo-Reyes, and
Crhistian Cisneros-Pérez, and
Laura Fuentes, and
Iván Moreno-Andrade, and
Elías Razo-Flores, and
Gonzalo Ruiz Filippi, and
Estela Tapia-Venegas, and
Javiera Toledo-Alarcón, and
Marcelo Zaiat
January 2017,
Asian-Australasian journal of animal sciences,
Claudia Etchebehere, and
Elena Castelló, and
Jorge Wenzel, and
Mélida del Pilar Anzola-Rojas, and
Liliana Borzacconi, and
Germán Buitrón, and
Lea Cabrol, and
Vivian María Carminato, and
Julian Carrillo-Reyes, and
Crhistian Cisneros-Pérez, and
Laura Fuentes, and
Iván Moreno-Andrade, and
Elías Razo-Flores, and
Gonzalo Ruiz Filippi, and
Estela Tapia-Venegas, and
Javiera Toledo-Alarcón, and
Marcelo Zaiat
September 2009,
Nature methods,
Claudia Etchebehere, and
Elena Castelló, and
Jorge Wenzel, and
Mélida del Pilar Anzola-Rojas, and
Liliana Borzacconi, and
Germán Buitrón, and
Lea Cabrol, and
Vivian María Carminato, and
Julian Carrillo-Reyes, and
Crhistian Cisneros-Pérez, and
Laura Fuentes, and
Iván Moreno-Andrade, and
Elías Razo-Flores, and
Gonzalo Ruiz Filippi, and
Estela Tapia-Venegas, and
Javiera Toledo-Alarcón, and
Marcelo Zaiat
August 2012,
Bioresource technology,
Claudia Etchebehere, and
Elena Castelló, and
Jorge Wenzel, and
Mélida del Pilar Anzola-Rojas, and
Liliana Borzacconi, and
Germán Buitrón, and
Lea Cabrol, and
Vivian María Carminato, and
Julian Carrillo-Reyes, and
Crhistian Cisneros-Pérez, and
Laura Fuentes, and
Iván Moreno-Andrade, and
Elías Razo-Flores, and
Gonzalo Ruiz Filippi, and
Estela Tapia-Venegas, and
Javiera Toledo-Alarcón, and
Marcelo Zaiat
January 2014,
PloS one,
Claudia Etchebehere, and
Elena Castelló, and
Jorge Wenzel, and
Mélida del Pilar Anzola-Rojas, and
Liliana Borzacconi, and
Germán Buitrón, and
Lea Cabrol, and
Vivian María Carminato, and
Julian Carrillo-Reyes, and
Crhistian Cisneros-Pérez, and
Laura Fuentes, and
Iván Moreno-Andrade, and
Elías Razo-Flores, and
Gonzalo Ruiz Filippi, and
Estela Tapia-Venegas, and
Javiera Toledo-Alarcón, and
Marcelo Zaiat
May 2014,
Antonie van Leeuwenhoek,
Claudia Etchebehere, and
Elena Castelló, and
Jorge Wenzel, and
Mélida del Pilar Anzola-Rojas, and
Liliana Borzacconi, and
Germán Buitrón, and
Lea Cabrol, and
Vivian María Carminato, and
Julian Carrillo-Reyes, and
Crhistian Cisneros-Pérez, and
Laura Fuentes, and
Iván Moreno-Andrade, and
Elías Razo-Flores, and
Gonzalo Ruiz Filippi, and
Estela Tapia-Venegas, and
Javiera Toledo-Alarcón, and
Marcelo Zaiat
April 2015,
Journal of environmental sciences (China),
Claudia Etchebehere, and
Elena Castelló, and
Jorge Wenzel, and
Mélida del Pilar Anzola-Rojas, and
Liliana Borzacconi, and
Germán Buitrón, and
Lea Cabrol, and
Vivian María Carminato, and
Julian Carrillo-Reyes, and
Crhistian Cisneros-Pérez, and
Laura Fuentes, and
Iván Moreno-Andrade, and
Elías Razo-Flores, and
Gonzalo Ruiz Filippi, and
Estela Tapia-Venegas, and
Javiera Toledo-Alarcón, and
Marcelo Zaiat
January 2020,
Frontiers in microbiology,
