BIOMAT Database Logo BIOMAT Database Logo

[Effects of paclitaxel loaded-drug micelles on cell proliferation and apoptosis of human lung cancer A549 cells]. 2015

Lin Wang, and Rui-shuang Yu, and Wen-liang Yang, and Shu-juan Luan, and Ben-kai Qin, and Xiao-bin Pang, and Guan-hua Du

This study was conducted to investigate the paclitaxel loaded by hydrazone bonds in poly(ethylene glycol)-poly(caprolactone) micelles (mPEG-PCL-PTX) on proliferation and apoptosis of human lung cancer A549 cells and its possible mechanisms of anti-tumor activity. The cell proliferation was measured with MTT assay. Flow cytometry were used to analyze the cell cycle. The cell apoptosis was analyzed using Hoechst/P staining. The expression levels of apoptotic genes expression in the mitochondrial apoptosis pathway were detected by RT-PCR and Western blotting, respectively. The mPEG-PCL-PTX could inhibit the proliferation of A549 cells and promote the apoptosis. The Bax, caspase-3 protein expression were increased while Bcl-2 protein expression was decreased in A549 cells. Results showed that the polymer containing hydrazone bond is non-toxic in vitro, the mPEG-PCL-PTX micelles can inhibit the proliferation and induce the apoptosis of A549 cells. Key words: paclitaxel; micelle; A549 cell; proliferation; cell cycle; apoptosis

UI MeSH Term Description Entries
D008175 Lung Neoplasms Tumors or cancer of the LUNG. Cancer of Lung,Lung Cancer,Pulmonary Cancer,Pulmonary Neoplasms,Cancer of the Lung,Neoplasms, Lung,Neoplasms, Pulmonary,Cancer, Lung,Cancer, Pulmonary,Cancers, Lung,Cancers, Pulmonary,Lung Cancers,Lung Neoplasm,Neoplasm, Lung,Neoplasm, Pulmonary,Pulmonary Cancers,Pulmonary Neoplasm
D008823 Micelles Particles consisting of aggregates of molecules held loosely together by secondary bonds. The surface of micelles are usually comprised of amphiphatic compounds that are oriented in a way that minimizes the energy of interaction between the micelle and its environment. Liquids that contain large numbers of suspended micelles are referred to as EMULSIONS. Micelle
D011091 Polyesters Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours. Polyester
D011092 Polyethylene Glycols Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS. Macrogols,Polyoxyethylenes,Carbowax,Macrogol,Polyethylene Glycol,Polyethylene Oxide,Polyethyleneoxide,Polyglycol,Glycol, Polyethylene,Glycols, Polyethylene,Oxide, Polyethylene,Oxides, Polyethylene,Polyethylene Oxides,Polyethyleneoxides,Polyglycols,Polyoxyethylene
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis
D017239 Paclitaxel A cyclodecane isolated from the bark of the Pacific yew tree, TAXUS BREVIFOLIA. It stabilizes MICROTUBULES in their polymerized form leading to cell death. 7-epi-Taxol,Anzatax,Bris Taxol,NSC-125973,Onxol,Paclitaxel, (4 alpha)-Isomer,Paxene,Praxel,Taxol,Taxol A,7 epi Taxol,NSC 125973,NSC125973,Taxol, Bris
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D049109 Cell Proliferation All of the processes involved in increasing CELL NUMBER including CELL DIVISION. Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular
D051028 bcl-2-Associated X Protein A member of the Bcl-2 protein family and homologous partner of C-BCL-2 PROTO-ONCOGENE PROTEIN. It regulates the release of CYTOCHROME C and APOPTOSIS INDUCING FACTOR from the MITOCHONDRIA. Several isoforms of BCL2-associated X protein occur due to ALTERNATIVE SPLICING of the mRNA for this protein. Bax Protein,Bax-alpha Protein,Bax-omega Protein,Bax-sigma Protein,Bax Apoptosis Regulator Protein,Bax-beta Protein,Bax-delta Protein,bcl2-Associated X Protein,bcl2-Associated X Protein Isoform alpha,bcl2-Associated X Protein Isoform beta,bcl2-Associated X Protein Isoform delta,bcl2-Associated X Protein Isoform omega,bcl2-Associated X Protein Isoform sigma,Bax alpha Protein,Bax beta Protein,Bax delta Protein,Bax omega Protein,Bax sigma Protein,Protein, bcl-2-Associated X,X Protein, bcl-2-Associated,bcl 2 Associated X Protein,bcl2 Associated X Protein,bcl2 Associated X Protein Isoform alpha,bcl2 Associated X Protein Isoform beta,bcl2 Associated X Protein Isoform delta,bcl2 Associated X Protein Isoform omega,bcl2 Associated X Protein Isoform sigma

Related Publications

Lin Wang, and Rui-shuang Yu, and Wen-liang Yang, and Shu-juan Luan, and Ben-kai Qin, and Xiao-bin Pang, and Guan-hua Du
Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells.
April 2018, Oncology letters,
Lin Wang, and Rui-shuang Yu, and Wen-liang Yang, and Shu-juan Luan, and Ben-kai Qin, and Xiao-bin Pang, and Guan-hua Du
Development of drug-loaded chitosan hollow nanoparticles for delivery of paclitaxel to human lung cancer A549 cells.
August 2017, Drug development and industrial pharmacy,
Lin Wang, and Rui-shuang Yu, and Wen-liang Yang, and Shu-juan Luan, and Ben-kai Qin, and Xiao-bin Pang, and Guan-hua Du
Oxymatrine inhibited cell proliferation by inducing apoptosis in human lung cancer A549 cells.
January 2015, Bio-medical materials and engineering,
Lin Wang, and Rui-shuang Yu, and Wen-liang Yang, and Shu-juan Luan, and Ben-kai Qin, and Xiao-bin Pang, and Guan-hua Du
[Effects of 17-AAG on the proliferation and apoptosis of human lung cancer A549 and H446 cells].
April 2015, Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases,
Lin Wang, and Rui-shuang Yu, and Wen-liang Yang, and Shu-juan Luan, and Ben-kai Qin, and Xiao-bin Pang, and Guan-hua Du
Effects of maslinic acid on the proliferation and apoptosis of A549 lung cancer cells.
January 2016, Molecular medicine reports,
Lin Wang, and Rui-shuang Yu, and Wen-liang Yang, and Shu-juan Luan, and Ben-kai Qin, and Xiao-bin Pang, and Guan-hua Du
Effect of evodiamine on the proliferation and apoptosis of A549 human lung cancer cells.
September 2016, Molecular medicine reports,
Lin Wang, and Rui-shuang Yu, and Wen-liang Yang, and Shu-juan Luan, and Ben-kai Qin, and Xiao-bin Pang, and Guan-hua Du
Effects of 2,2',4'-trihydroxychalcone on the proliferation, metastasis and apoptosis of A549 human lung cancer cells.
April 2022, Oncology letters,
Lin Wang, and Rui-shuang Yu, and Wen-liang Yang, and Shu-juan Luan, and Ben-kai Qin, and Xiao-bin Pang, and Guan-hua Du
[Effects of GM3 on proliferation, apoptosis and VEGF expression in human lung adenocarcinoma cell line A549 cells].
April 2011, Zhonghua zhong liu za zhi [Chinese journal of oncology],
Lin Wang, and Rui-shuang Yu, and Wen-liang Yang, and Shu-juan Luan, and Ben-kai Qin, and Xiao-bin Pang, and Guan-hua Du
Deguelin and Paclitaxel Loaded PEG-PCL Nano-Micelles for Suppressing the Proliferation and Inducing Apoptosis of Breast Cancer Cells.
February 2024, Frontiers in bioscience (Landmark edition),
Lin Wang, and Rui-shuang Yu, and Wen-liang Yang, and Shu-juan Luan, and Ben-kai Qin, and Xiao-bin Pang, and Guan-hua Du
UNBS5162 inhibits the proliferation of human A549 non-small-cell lung cancer cells by promoting apoptosis.
January 2018, Thoracic cancer,
Copied contents to your clipboard!