A response of limited turbidostat S. cerevisiae 14 culture on rapid increase in temperature from optimal 30 degrees to supraoptimal 37.5 degrees C. The temporal thermotolerance was absent in glucose and phosphate-limited cultures. Limitation of nitrogen, Mg, betaalanine, biotin, and, to a certain extent, potassium did not decrease the thermotolerance. The pattern of changes in age composition and specific optical density was found to be similar in limited and unlimited cultures. The glucose and phosphate expenditures at 37.5 degrees C were estimated. The response to heat shock was found to depend on the nature of the limiting factor and the extent of limiting.