BIOMAT Database Logo BIOMAT Database Logo

Assessment of conjunctival goblet cell density using laser scanning confocal microscopy versus impression cytology. 2016

Luisa H Colorado, and Yahya Alzahrani, and Nicola Pritchard, and Nathan Efron
Institute of Health and Biomedical Innovation, and School of Optometry and Vision Science, Queensland University of Technology, Brisbane, Australia.

OBJECTIVE To determine the association between conjunctival goblet cell density (GCD) assessed using in vivo laser scanning confocal microscopy and conjunctival impression cytology in a healthy population. METHODS Ninety (90) healthy participants undertook a validated 5-item dry eye questionnaire, non-invasive tear film break-up time measurement, ocular surface fluorescein staining and phenol red thread test. These tests where undertaken to diagnose and exclude participants with dry eye. The nasal bulbar conjunctiva was imaged using laser scanning confocal microscopy (LSCM). Conjunctival impression cytology (CIC) was performed in the same region a few minutes later. Conjunctival goblet cell density was calculated as cells/mm(2). RESULTS There was a strong positive correlation of conjunctival GCD between LSCM and CIC (ρ=0.66). Conjunctival goblet cell density was 475±41 cells/mm(2) and 466±51 cells/mm(2) measured by LSCM and CIC, respectively. CONCLUSIONS The strong association between in vivo and in vitro cellular analysis for measuring conjunctival GCD suggests that the more invasive CIC can be replaced by the less invasive LSCM in research and clinical practice.

UI MeSH Term Description Entries
D008297 Male Males
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D002452 Cell Count The number of CELLS of a specific kind, usually measured per unit volume or area of sample. Cell Density,Cell Number,Cell Counts,Cell Densities,Cell Numbers,Count, Cell,Counts, Cell,Densities, Cell,Density, Cell,Number, Cell,Numbers, Cell
D003228 Conjunctiva The mucous membrane that covers the posterior surface of the eyelids and the anterior pericorneal surface of the eyeball. Bulbar Conjunctiva,Palpebral Conjunctiva,Plica Semilunaris of Conjunctiva,Plicae Semilunares of Conjunctiva,Tunica Conjunctiva,Conjunctiva, Bulbar,Conjunctiva, Palpebral,Conjunctivas
D003941 Diagnostic Techniques, Ophthalmological Methods and procedures for the diagnosis of diseases of the eye or of vision disorders. Diagnostic Technic, Ophthalmological,Diagnostic Technics, Ophthalmologic,Diagnostic Technics, Ophthalmological,Diagnostic Technique, Ophthalmological,Diagnostic Techniques, Ophthalmologic,Ophthalmological Diagnostic Technic,Ophthalmological Diagnostic Technics,Ophthalmological Diagnostic Technique,Ophthalmological Diagnostic Techniques,Technic, Ophthalmological Diagnostic,Technics, Ophthalmological Diagnostic,Technique, Ophthalmological Diagnostic,Techniques, Ophthalmological Diagnostic,Diagnostic Technic, Ophthalmologic,Diagnostic Technique, Ophthalmologic,Ophthalmologic Diagnostic Technic,Ophthalmologic Diagnostic Technics,Ophthalmologic Diagnostic Technique,Ophthalmologic Diagnostic Techniques,Technic, Ophthalmologic Diagnostic,Technics, Ophthalmologic Diagnostic,Technique, Ophthalmologic Diagnostic,Techniques, Ophthalmologic Diagnostic
D005260 Female Females
D006652 Histological Techniques Methods of preparing tissue for examination and study of the origin, structure, function, or pathology. Histologic Technic,Histologic Technics,Histologic Technique,Histologic Techniques,Histological Technics,Technic, Histologic,Technics, Histologic,Technique, Histologic,Techniques, Histologic,Histological Technic,Histological Technique,Technic, Histological,Technics, Histological,Technique, Histological,Techniques, Histological
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D001706 Biopsy Removal and pathologic examination of specimens from the living body. Biopsies

Related Publications

Luisa H Colorado, and Yahya Alzahrani, and Nicola Pritchard, and Nathan Efron
In vivo evaluation of superior limbic keratoconjunctivitis using laser scanning confocal microscopy and conjunctival impression cytology.
August 2010, Investigative ophthalmology & visual science,
Luisa H Colorado, and Yahya Alzahrani, and Nicola Pritchard, and Nathan Efron
Sampling area selection for the assessment of goblet cell density from conjunctival impression cytology specimens.
March 2012, Eye & contact lens,
Luisa H Colorado, and Yahya Alzahrani, and Nicola Pritchard, and Nathan Efron
Conjunctival goblet cells density and preservative-free tafluprost therapy for glaucoma: an in vivo confocal microscopy and impression cytology study.
August 2013, Acta ophthalmologica,
Luisa H Colorado, and Yahya Alzahrani, and Nicola Pritchard, and Nathan Efron
In Vivo Scanning Laser Confocal Microscopy of Conjunctival Goblet Cells in Medically-controlled Glaucoma.
January 2018, In vivo (Athens, Greece),
Luisa H Colorado, and Yahya Alzahrani, and Nicola Pritchard, and Nathan Efron
Assessment of goblet cell size and density in relation to epithelial cell (multi)layering on conjunctival impression cytology samples.
August 2020, Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie,
Luisa H Colorado, and Yahya Alzahrani, and Nicola Pritchard, and Nathan Efron
Laser scanning confocal microscopy for conjunctival epithelium imaging.
January 2010, Developments in ophthalmology,
Luisa H Colorado, and Yahya Alzahrani, and Nicola Pritchard, and Nathan Efron
[In vivo imaging of the conjunctival epithelium using confocal laser scanning microscopy].
May 2006, Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft,
Luisa H Colorado, and Yahya Alzahrani, and Nicola Pritchard, and Nathan Efron
Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC).
August 2009, Molecular vision,
Luisa H Colorado, and Yahya Alzahrani, and Nicola Pritchard, and Nathan Efron
Characterization of Goblet Cells in a Pterygium Biopsy Using Laser Scanning Confocal Microscopy and Immunohistochemistry.
August 2016, Cornea,
Luisa H Colorado, and Yahya Alzahrani, and Nicola Pritchard, and Nathan Efron
[Intraoral diagnostics using confocal laser scanning microscopy].
February 2009, Biomedizinische Technik. Biomedical engineering,
Copied contents to your clipboard!