Crystallization and preliminary X-ray studies of an aspartate aminotransferase mutant from Escherichia coli. 1989

J Jäger, and E Köhler, and P Tucker, and U Sauder, and Z Housley-Markovic, and I Fotheringham, and M Edwards, and M Hunter, and K Kirschner, and J N Jansonius
Department of Structural Biology, University of Basel, Switzerland.

Mutant aspartate aminotransferase V39L (Val39 replaced by Leu) from Escherichia coli has been crystallized into a monoclinic cell from a polyethylene glycol solution (pH 7.5) by vapor diffusion. The space group and the unit cell dimensions have been determined using a precession camera, a CAD4 diffractometer and a Nicolet Xentronics area detector to be P2(1) with a = 86.8 A, b = 79.9 A, c = 89.4 A, beta = 118.74 degrees. The crystals diffract to better than 2.3 A and are suitable for X-ray structure analysis.

UI MeSH Term Description Entries
D008958 Models, Molecular Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures. Molecular Models,Model, Molecular,Molecular Model
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D003460 Crystallization The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D001219 Aspartate Aminotransferases Enzymes of the transferase class that catalyze the conversion of L-aspartate and 2-ketoglutarate to oxaloacetate and L-glutamate. EC 2.6.1.1. Aspartate Aminotransferase,Aspartate Transaminase,Glutamic-Oxaloacetic Transaminase,SGOT,Aspartate Apoaminotransferase,Glutamate-Aspartate Transaminase,L-Aspartate-2-Oxoglutarate Aminotransferase,Serum Glutamic-Oxaloacetic Transaminase,Aminotransferase, Aspartate,Aminotransferase, L-Aspartate-2-Oxoglutarate,Aminotransferases, Aspartate,Apoaminotransferase, Aspartate,Glutamate Aspartate Transaminase,Glutamic Oxaloacetic Transaminase,Glutamic-Oxaloacetic Transaminase, Serum,L Aspartate 2 Oxoglutarate Aminotransferase,Serum Glutamic Oxaloacetic Transaminase,Transaminase, Aspartate,Transaminase, Glutamate-Aspartate,Transaminase, Glutamic-Oxaloacetic,Transaminase, Serum Glutamic-Oxaloacetic
D014961 X-Ray Diffraction The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Xray Diffraction,Diffraction, X-Ray,Diffraction, Xray,Diffractions, X-Ray,Diffractions, Xray,X Ray Diffraction,X-Ray Diffractions,Xray Diffractions

Related Publications

J Jäger, and E Köhler, and P Tucker, and U Sauder, and Z Housley-Markovic, and I Fotheringham, and M Edwards, and M Hunter, and K Kirschner, and J N Jansonius
May 1981, The Journal of biological chemistry,
J Jäger, and E Köhler, and P Tucker, and U Sauder, and Z Housley-Markovic, and I Fotheringham, and M Edwards, and M Hunter, and K Kirschner, and J N Jansonius
September 1986, Journal of molecular biology,
J Jäger, and E Köhler, and P Tucker, and U Sauder, and Z Housley-Markovic, and I Fotheringham, and M Edwards, and M Hunter, and K Kirschner, and J N Jansonius
March 1987, Journal of biochemistry,
J Jäger, and E Köhler, and P Tucker, and U Sauder, and Z Housley-Markovic, and I Fotheringham, and M Edwards, and M Hunter, and K Kirschner, and J N Jansonius
January 2011, Acta crystallographica. Section F, Structural biology and crystallization communications,
J Jäger, and E Köhler, and P Tucker, and U Sauder, and Z Housley-Markovic, and I Fotheringham, and M Edwards, and M Hunter, and K Kirschner, and J N Jansonius
January 2008, Acta crystallographica. Section F, Structural biology and crystallization communications,
J Jäger, and E Köhler, and P Tucker, and U Sauder, and Z Housley-Markovic, and I Fotheringham, and M Edwards, and M Hunter, and K Kirschner, and J N Jansonius
December 1993, Journal of molecular biology,
J Jäger, and E Köhler, and P Tucker, and U Sauder, and Z Housley-Markovic, and I Fotheringham, and M Edwards, and M Hunter, and K Kirschner, and J N Jansonius
May 1989, Journal of biochemistry,
J Jäger, and E Köhler, and P Tucker, and U Sauder, and Z Housley-Markovic, and I Fotheringham, and M Edwards, and M Hunter, and K Kirschner, and J N Jansonius
July 1998, Acta crystallographica. Section D, Biological crystallography,
J Jäger, and E Köhler, and P Tucker, and U Sauder, and Z Housley-Markovic, and I Fotheringham, and M Edwards, and M Hunter, and K Kirschner, and J N Jansonius
August 1990, Journal of molecular biology,
J Jäger, and E Köhler, and P Tucker, and U Sauder, and Z Housley-Markovic, and I Fotheringham, and M Edwards, and M Hunter, and K Kirschner, and J N Jansonius
October 1989, Journal of molecular biology,
Copied contents to your clipboard!