Pronuclear epigenetic modification of protamine deficient human sperm following injection into mouse oocytes. 2016

Hoda Rajabi, and Homa Mohseni-Kouchesfehani, and Abdollah Mohammadi-Sangcheshmeh, and Fattaneh Farifteh-Nobijari, and Mohammad Salehi
a Stem Cell Technology Research Center , Tehran , Iran.

Epigenetic abnormalities and abnormal chromatin structure in sperm may lead to male infertility. Protamine deficiency is among the disorders of chromatin structure in sperm. The study of epigenetic changes in male pronuclei is necessary since abnormal sperm is sometimes used to create embryos using assisted reproductive techniques. The present study was carried out to compare epigenetic global marks in male pronuclei derived from normal and protamine deficient sperm cells. To do so, interspecies fertilization was used to obtain the male pronucleus. Normal and protamine deficient sperm cells, which were identified by chromomycin A3 staining, were injected into mouse oocytes. Oocytes were cultured until pronuclear formation and were then labeled with different antibodies (anti 5-methylcytosine, anti 5-hydroxymethylcytosine, and anti acetyl H4K12). Based on the fluorescence intensity, the level of each of these epigenetic factors was determined and they revealed a significant relationship between the level of sperm protamine deficiency and sperm epigenetic factors. Protamine deficiency was found to be associated with an increased methylation (p=0) and decreased hydroxymethylation rate (p=0.015) of the male pronucleus chromatin. However, no association was found between protamine deficiency and the level of H4K12 acetylation (p=0.548). Also, the efficiency of fertilization in protamine deficient sperm cells was less than normal. These results suggest that protamine deficient sperm cells lead to the formation of epigenetically altered pronuclei.

UI MeSH Term Description Entries
D008297 Male Males
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D011479 Protamines A group of simple proteins that yield basic amino acids on hydrolysis and that occur combined with nucleic acid in the sperm of fish. Protamines contain very few kinds of amino acids. Protamine sulfate combines with heparin to form a stable inactive complex; it is used to neutralize the anticoagulant action of heparin in the treatment of heparin overdose. (From Merck Index, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p692) Protamine,Protamine Sulfate,Protamine Chloride,Chloride, Protamine,Sulfate, Protamine
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D005260 Female Females
D005306 Fertilization The fusion of a spermatozoon (SPERMATOZOA) with an OVUM thus resulting in the formation of a ZYGOTE. Conception,Fertilization, Delayed,Fertilization, Polyspermic,Conceptions,Delayed Fertilization,Delayed Fertilizations,Fertilizations,Fertilizations, Delayed,Fertilizations, Polyspermic,Polyspermic Fertilization,Polyspermic Fertilizations
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013094 Spermatozoa Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility. Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms
D044127 Epigenesis, Genetic A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression. Epigenetic Processes,Epigenetic Process,Epigenetics Processes,Genetic Epigenesis,Process, Epigenetic,Processes, Epigenetic,Processes, Epigenetics

Related Publications

Hoda Rajabi, and Homa Mohseni-Kouchesfehani, and Abdollah Mohammadi-Sangcheshmeh, and Fattaneh Farifteh-Nobijari, and Mohammad Salehi
May 2008, Genomics,
Hoda Rajabi, and Homa Mohseni-Kouchesfehani, and Abdollah Mohammadi-Sangcheshmeh, and Fattaneh Farifteh-Nobijari, and Mohammad Salehi
May 2002, Zygote (Cambridge, England),
Hoda Rajabi, and Homa Mohseni-Kouchesfehani, and Abdollah Mohammadi-Sangcheshmeh, and Fattaneh Farifteh-Nobijari, and Mohammad Salehi
August 2011, Protein and peptide letters,
Hoda Rajabi, and Homa Mohseni-Kouchesfehani, and Abdollah Mohammadi-Sangcheshmeh, and Fattaneh Farifteh-Nobijari, and Mohammad Salehi
March 2008, Human reproduction (Oxford, England),
Hoda Rajabi, and Homa Mohseni-Kouchesfehani, and Abdollah Mohammadi-Sangcheshmeh, and Fattaneh Farifteh-Nobijari, and Mohammad Salehi
March 2005, Genetika,
Hoda Rajabi, and Homa Mohseni-Kouchesfehani, and Abdollah Mohammadi-Sangcheshmeh, and Fattaneh Farifteh-Nobijari, and Mohammad Salehi
May 2002, Zygote (Cambridge, England),
Hoda Rajabi, and Homa Mohseni-Kouchesfehani, and Abdollah Mohammadi-Sangcheshmeh, and Fattaneh Farifteh-Nobijari, and Mohammad Salehi
October 2003, Reproduction in domestic animals = Zuchthygiene,
Hoda Rajabi, and Homa Mohseni-Kouchesfehani, and Abdollah Mohammadi-Sangcheshmeh, and Fattaneh Farifteh-Nobijari, and Mohammad Salehi
April 2011, Journal of assisted reproduction and genetics,
Hoda Rajabi, and Homa Mohseni-Kouchesfehani, and Abdollah Mohammadi-Sangcheshmeh, and Fattaneh Farifteh-Nobijari, and Mohammad Salehi
October 2009, Animal reproduction science,
Hoda Rajabi, and Homa Mohseni-Kouchesfehani, and Abdollah Mohammadi-Sangcheshmeh, and Fattaneh Farifteh-Nobijari, and Mohammad Salehi
January 2008, Methods in molecular biology (Clifton, N.J.),
Copied contents to your clipboard!