Intracellular delivery of antibodies by chimeric Sesbania mosaic virus (SeMV) virus like particles. 2016

Ambily Abraham, and Usha Natraj, and Anjali A Karande, and Ashutosh Gulati, and Mathur R N Murthy, and Sathyabalan Murugesan, and Pavithra Mukunda, and Handanahal S Savithri
Department of Biochemistry, Indian Institute of Science, Karnataka, India.

The therapeutic potential of antibodies has not been fully exploited as they fail to cross cell membrane. In this article, we have tested the possibility of using plant virus based nanoparticles for intracellular delivery of antibodies. For this purpose, Sesbania mosaic virus coat protein (CP) was genetically engineered with the B domain of Staphylococcus aureus protein A (SpA) at the βH-βI loop, to generate SeMV loop B (SLB), which self-assembled to virus like particles (VLPs) with 43 times higher affinity towards antibodies. CP and SLB could internalize into various types of mammalian cells and SLB could efficiently deliver three different monoclonal antibodies-D6F10 (targeting abrin), anti-α-tubulin (targeting intracellular tubulin) and Herclon (against HER2 receptor) inside the cells. Such a mode of delivery was much more effective than antibodies alone treatment. These results highlight the potential of SLB as a universal nanocarrier for intracellular delivery of antibodies.

UI MeSH Term Description Entries
D008546 Melanoma, Experimental Experimentally induced tumor that produces MELANIN in animals to provide a model for studying human MELANOMA. B16 Melanoma,Melanoma, B16,Melanoma, Cloudman S91,Melanoma, Harding-Passey,Experimental Melanoma,Experimental Melanomas,Harding Passey Melanoma,Melanomas, Experimental,B16 Melanomas,Cloudman S91 Melanoma,Harding-Passey Melanoma,Melanoma, Harding Passey,Melanomas, B16,S91 Melanoma, Cloudman
D009029 Mosaic Viruses Viruses which produce a mottled appearance of the leaves of plants. Mosaic Virus,Virus, Mosaic,Viruses, Mosaic
D011993 Recombinant Fusion Proteins Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes. Fusion Proteins, Recombinant,Recombinant Chimeric Protein,Recombinant Fusion Protein,Recombinant Hybrid Protein,Chimeric Proteins, Recombinant,Hybrid Proteins, Recombinant,Recombinant Chimeric Proteins,Recombinant Hybrid Proteins,Chimeric Protein, Recombinant,Fusion Protein, Recombinant,Hybrid Protein, Recombinant,Protein, Recombinant Chimeric,Protein, Recombinant Fusion,Protein, Recombinant Hybrid,Proteins, Recombinant Chimeric,Proteins, Recombinant Fusion,Proteins, Recombinant Hybrid
D004337 Drug Carriers Forms to which substances are incorporated to improve the delivery and the effectiveness of drugs. Drug carriers are used in drug-delivery systems such as the controlled-release technology to prolong in vivo drug actions, decrease drug metabolism, and reduce drug toxicity. Carriers are also used in designs to increase the effectiveness of drug delivery to the target sites of pharmacological actions. Liposomes, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, and carrier erythrocytes among others have been employed as biodegradable drug carriers. Drug Carrier
D004353 Drug Evaluation, Preclinical Preclinical testing of drugs in experimental animals or in vitro for their biological and toxic effects and potential clinical applications. Drug Screening,Evaluation Studies, Drug, Pre-Clinical,Drug Evaluation Studies, Preclinical,Drug Evaluations, Preclinical,Evaluation Studies, Drug, Preclinical,Evaluation, Preclinical Drug,Evaluations, Preclinical Drug,Medicinal Plants Testing, Preclinical,Preclinical Drug Evaluation,Preclinical Drug Evaluations,Drug Screenings,Screening, Drug,Screenings, Drug
D006367 HeLa Cells The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays. Cell, HeLa,Cells, HeLa,HeLa Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D013205 Staphylococcal Protein A A protein present in the cell wall of most Staphylococcus aureus strains. The protein selectively binds to the Fc region of human normal and myeloma-derived IMMUNOGLOBULIN G. It elicits antibody activity and may cause hypersensitivity reactions due to histamine release; has also been used as cell surface antigen marker and in the clinical assessment of B lymphocyte function. Protein A,Protein A, Staphylococcal

Related Publications

Ambily Abraham, and Usha Natraj, and Anjali A Karande, and Ashutosh Gulati, and Mathur R N Murthy, and Sathyabalan Murugesan, and Pavithra Mukunda, and Handanahal S Savithri
January 2014, FEBS open bio,
Ambily Abraham, and Usha Natraj, and Anjali A Karande, and Ashutosh Gulati, and Mathur R N Murthy, and Sathyabalan Murugesan, and Pavithra Mukunda, and Handanahal S Savithri
November 1992, Proceedings of the National Academy of Sciences of the United States of America,
Ambily Abraham, and Usha Natraj, and Anjali A Karande, and Ashutosh Gulati, and Mathur R N Murthy, and Sathyabalan Murugesan, and Pavithra Mukunda, and Handanahal S Savithri
November 2018, Biochemical and biophysical research communications,
Ambily Abraham, and Usha Natraj, and Anjali A Karande, and Ashutosh Gulati, and Mathur R N Murthy, and Sathyabalan Murugesan, and Pavithra Mukunda, and Handanahal S Savithri
August 2023, Journal of materials chemistry. B,
Ambily Abraham, and Usha Natraj, and Anjali A Karande, and Ashutosh Gulati, and Mathur R N Murthy, and Sathyabalan Murugesan, and Pavithra Mukunda, and Handanahal S Savithri
March 1999, Biological chemistry,
Ambily Abraham, and Usha Natraj, and Anjali A Karande, and Ashutosh Gulati, and Mathur R N Murthy, and Sathyabalan Murugesan, and Pavithra Mukunda, and Handanahal S Savithri
February 2019, Veterinary microbiology,
Ambily Abraham, and Usha Natraj, and Anjali A Karande, and Ashutosh Gulati, and Mathur R N Murthy, and Sathyabalan Murugesan, and Pavithra Mukunda, and Handanahal S Savithri
January 2012, PloS one,
Ambily Abraham, and Usha Natraj, and Anjali A Karande, and Ashutosh Gulati, and Mathur R N Murthy, and Sathyabalan Murugesan, and Pavithra Mukunda, and Handanahal S Savithri
July 2021, Vaccine,
Ambily Abraham, and Usha Natraj, and Anjali A Karande, and Ashutosh Gulati, and Mathur R N Murthy, and Sathyabalan Murugesan, and Pavithra Mukunda, and Handanahal S Savithri
May 2018, ACS nano,
Ambily Abraham, and Usha Natraj, and Anjali A Karande, and Ashutosh Gulati, and Mathur R N Murthy, and Sathyabalan Murugesan, and Pavithra Mukunda, and Handanahal S Savithri
August 2022, Viruses,
Copied contents to your clipboard!