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BACKGROUND Cytomegalovirus (CMV) infection is frequent in HIV adults. It is unknown usefulness of quantitative methods for diagnosing the CMV disease in Chilean patients. OBJECTIVE To determine the performance of antigenemia and real time polymerase chain reaction (rtPCR) in the diagnosis of CMV disease in Chilean HIV adults. METHODS Detection of CMV by viral isolation (AVR), antigenemia and quantitative rtPCR in HIV adults. RESULTS The 102 adults with suspected CMV disease had lower LTCD4 count and higher HIV viral load than 77 patients without suspicion (p < 0.05). Antigenemia and PCR were positive in 47 (46.1%) and 37 (36.3%) adults with clinical suspicion and in 2 (2.6%) and 4 (5.2%) of 77 without suspicion. The sensitivity, specificity, positive and negative predictive value of antigenemia and RPCtr were 92%, 80%, 72% and 95% and 72%, 95%, 92% and 80%, respectively. The cutoff values were ≥ lcell (+) and ≥ 5.5 log10 copies/2 x 10(6) cells. CMV was isolated in 6/179 patients (3.4%), all symptomatic. CONCLUSIONS Positivity of antigenemia and rtPCR are similar for diagnosing CMV disease in Chilean HIV adults. AVR is inappropriate as a gold standard for its low performance.
Vivían Luchsinger, and
Patricia Vásquez, and
Matías Silva, and
M José Bruno, and
Izkia Siches, and
Javier Villarroel, and
M Luisa Garmendia, and
Carmen Larrañaga
April 2011,
Revista chilena de infectologia : organo oficial de la Sociedad Chilena de Infectologia,
Vivían Luchsinger, and
Patricia Vásquez, and
Matías Silva, and
M José Bruno, and
Izkia Siches, and
Javier Villarroel, and
M Luisa Garmendia, and
Carmen Larrañaga
March 1997,
AIDS (London, England),
Vivían Luchsinger, and
Patricia Vásquez, and
Matías Silva, and
M José Bruno, and
Izkia Siches, and
Javier Villarroel, and
M Luisa Garmendia, and
Carmen Larrañaga
November 2018,
Indian journal of ophthalmology,
Vivían Luchsinger, and
Patricia Vásquez, and
Matías Silva, and
M José Bruno, and
Izkia Siches, and
Javier Villarroel, and
M Luisa Garmendia, and
Carmen Larrañaga
December 1999,
The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases,
Vivían Luchsinger, and
Patricia Vásquez, and
Matías Silva, and
M José Bruno, and
Izkia Siches, and
Javier Villarroel, and
M Luisa Garmendia, and
Carmen Larrañaga
September 2019,
American journal of ophthalmology case reports,
Vivían Luchsinger, and
Patricia Vásquez, and
Matías Silva, and
M José Bruno, and
Izkia Siches, and
Javier Villarroel, and
M Luisa Garmendia, and
Carmen Larrañaga
October 2009,
Diagnostic microbiology and infectious disease,
Vivían Luchsinger, and
Patricia Vásquez, and
Matías Silva, and
M José Bruno, and
Izkia Siches, and
Javier Villarroel, and
M Luisa Garmendia, and
Carmen Larrañaga
December 2005,
Klinicka mikrobiologie a infekcni lekarstvi,
Vivían Luchsinger, and
Patricia Vásquez, and
Matías Silva, and
M José Bruno, and
Izkia Siches, and
Javier Villarroel, and
M Luisa Garmendia, and
Carmen Larrañaga
January 1998,
Voprosy virusologii,
Vivían Luchsinger, and
Patricia Vásquez, and
Matías Silva, and
M José Bruno, and
Izkia Siches, and
Javier Villarroel, and
M Luisa Garmendia, and
Carmen Larrañaga
March 2012,
Zhonghua yi xue za zhi,
Vivían Luchsinger, and
Patricia Vásquez, and
Matías Silva, and
M José Bruno, and
Izkia Siches, and
Javier Villarroel, and
M Luisa Garmendia, and
Carmen Larrañaga
April 2002,
Bone marrow transplantation,
