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An enzyme-linked immunosorbent assay (ELISA) for circulating IgG mouse antibody to Plasmodium falciparum circumsporozoite (CS) protein was modified for use with human sera collected in an area of northern Zambia that was endemic for malaria and from individuals never exposed to malaria. Optimum sensitivity was achieved using Immulon 2 microtitration plates, boiled casein-Tween 20 blocking buffer, and by adding a solution of boiled casein (4 micrograms/ml) to the capture antigen diluent. The results for the detection of anti-CS IgG correlated well with those of sporozoite immunofluorescence antibody assays. Modification of the ELISA method permitted the simultaneous detection of anti-CS IgG and IgM antibody on a single serum sample in the same well of the microtitration plate and the detection of anti-CS IgG antibody in Kenyan dried whole-blood samples collected on filter-paper. The assay has been used to monitor human antibody levels in a phase-I malaria vaccine trial and in longitudinal studies of malaria transmission in Thailand and Kenya.
R A Wirtz, and
J F Duncan, and
E K Njelesani, and
I Schneider, and
A E Brown, and
C N Oster, and
J B Were, and
H K Webster
December 1993,
The Journal of parasitology,
R A Wirtz, and
J F Duncan, and
E K Njelesani, and
I Schneider, and
A E Brown, and
C N Oster, and
J B Were, and
H K Webster
October 1992,
The American journal of tropical medicine and hygiene,
R A Wirtz, and
J F Duncan, and
E K Njelesani, and
I Schneider, and
A E Brown, and
C N Oster, and
J B Were, and
H K Webster
June 1992,
Experimental parasitology,
R A Wirtz, and
J F Duncan, and
E K Njelesani, and
I Schneider, and
A E Brown, and
C N Oster, and
J B Were, and
H K Webster
February 2020,
Journal of molecular biology,
R A Wirtz, and
J F Duncan, and
E K Njelesani, and
I Schneider, and
A E Brown, and
C N Oster, and
J B Were, and
H K Webster
November 2017,
Proceedings of the National Academy of Sciences of the United States of America,
R A Wirtz, and
J F Duncan, and
E K Njelesani, and
I Schneider, and
A E Brown, and
C N Oster, and
J B Were, and
H K Webster
August 2017,
Antibodies (Basel, Switzerland),
R A Wirtz, and
J F Duncan, and
E K Njelesani, and
I Schneider, and
A E Brown, and
C N Oster, and
J B Were, and
H K Webster
June 1990,
The Southeast Asian journal of tropical medicine and public health,
R A Wirtz, and
J F Duncan, and
E K Njelesani, and
I Schneider, and
A E Brown, and
C N Oster, and
J B Were, and
H K Webster
December 1987,
Experimental parasitology,
R A Wirtz, and
J F Duncan, and
E K Njelesani, and
I Schneider, and
A E Brown, and
C N Oster, and
J B Were, and
H K Webster
May 2002,
Experimental parasitology,
R A Wirtz, and
J F Duncan, and
E K Njelesani, and
I Schneider, and
A E Brown, and
C N Oster, and
J B Were, and
H K Webster
September 2021,
Malaria journal,
