Biomaterial Database

Salt-Responsive Transcriptome Profiling of Suaeda glauca via RNA Sequencing. 2016

Hangxia Jin, and Dekun Dong, and Qinghua Yang, and Danhua Zhu

Zhejiang Academy of Agricultural Science, Institute of Crops and Nuclear Technology Utilization, Hangzhou Zhejiang 310021, People's Republic of China.

BACKGROUND Suaeda glauca, a succulent halophyte of the Chenopodiaceae family, is widely distributed in coastal areas of China. Suaeda glauca is highly resistant to salt and alkali stresses. In the present study, the salt-responsive transcriptome of Suaeda glauca was analyzed to identify genes involved in salt tolerance and study halophilic mechanisms in this halophyte. RESULTS Illumina HiSeq 2500 was used to sequence cDNA libraries from salt-treated and control samples with three replicates each treatment. De novo assembly of the six transcriptomes identified 75,445 unigenes. A total of 23,901 (31.68%) unigenes were annotated. Compared with transcriptomes from the three salt-treated and three salt-free samples, 231 differentially expressed genes (DEGs) were detected (including 130 up-regulated genes and 101 down-regulated genes), and 195 unigenes were functionally annotated. Based on the Gene Ontology (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) classifications of the DEGs, more attention should be paid to transcripts associated with signal transduction, transporters, the cell wall and growth, defense metabolism and transcription factors involved in salt tolerance. CONCLUSIONS This report provides a genome-wide transcriptional analysis of a halophyte, Suaeda glauca, under salt stress. Further studies of the genetic basis of salt tolerance in halophytes are warranted.

UI	MeSH Term	Description	Entries
D015723	Gene Library	A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.	DNA Library,cDNA Library,DNA Libraries,Gene Libraries,Libraries, DNA,Libraries, Gene,Libraries, cDNA,Library, DNA,Library, Gene,Library, cDNA,cDNA Libraries
D017423	Sequence Analysis, RNA	A multistage process that includes cloning, physical mapping, subcloning, sequencing, and information analysis of an RNA SEQUENCE.	RNA Sequence Analysis,Sequence Determination, RNA,Analysis, RNA Sequence,Determination, RNA Sequence,Determinations, RNA Sequence,RNA Sequence Determination,RNA Sequence Determinations,RNA Sequencing,Sequence Determinations, RNA,Analyses, RNA Sequence,RNA Sequence Analyses,Sequence Analyses, RNA,Sequencing, RNA
D055051	Salt-Tolerant Plants	Plants that can grow well in soils that have a high SALINITY.	Halophytes,Plants, Salt-Tolerant,Halophilic Plants,Halophytic Plants,Saline-Resistant Plants,Saline-Tolerant Plants,Salt-Adapted Plants,Salt-Resistant Plants,Halophilic Plant,Halophyte,Halophytic Plant,Plant, Halophilic,Plant, Halophytic,Plant, Saline-Resistant,Plant, Saline-Tolerant,Plant, Salt-Adapted,Plant, Salt-Resistant,Plant, Salt-Tolerant,Plants, Halophilic,Plants, Halophytic,Plants, Saline-Resistant,Plants, Saline-Tolerant,Plants, Salt Tolerant,Plants, Salt-Adapted,Plants, Salt-Resistant,Saline Resistant Plants,Saline Tolerant Plants,Saline-Resistant Plant,Saline-Tolerant Plant,Salt Adapted Plants,Salt Resistant Plants,Salt Tolerant Plants,Salt-Adapted Plant,Salt-Resistant Plant,Salt-Tolerant Plant
D060888	Real-Time Polymerase Chain Reaction	Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.	Kinetic Polymerase Chain Reaction,Quantitative Real-Time PCR,Quantitative Real-Time Polymerase Chain Reaction,Real-Time PCR,PCR, Quantitative Real-Time,PCR, Real-Time,PCRs, Quantitative Real-Time,PCRs, Real-Time,Quantitative Real Time PCR,Quantitative Real Time Polymerase Chain Reaction,Quantitative Real-Time PCRs,Real Time PCR,Real Time Polymerase Chain Reaction,Real-Time PCR, Quantitative,Real-Time PCRs,Real-Time PCRs, Quantitative
D018506	Gene Expression Regulation, Plant	Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.	Plant Gene Expression Regulation,Regulation of Gene Expression, Plant,Regulation, Gene Expression, Plant
D018744	DNA, Plant	Deoxyribonucleic acid that makes up the genetic material of plants.	Plant DNA
D019663	Chenopodiaceae	The goosefoot plant family of the order Caryophyllales, subclass Caryophyllidae, class Magnoliopsida. It includes beets and chard (BETA VULGARIS), as well as SPINACH, and salt tolerant plants.	Halogeton,Kochia,Salicornia,Suaeda,Halogetons,Kochias,Salicornias,Suaedas
D020869	Gene Expression Profiling	The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.	Gene Expression Analysis,Gene Expression Pattern Analysis,Transcript Expression Analysis,Transcriptome Profiling,Transcriptomics,mRNA Differential Display,Gene Expression Monitoring,Transcriptome Analysis,Analyses, Gene Expression,Analyses, Transcript Expression,Analyses, Transcriptome,Analysis, Gene Expression,Analysis, Transcript Expression,Analysis, Transcriptome,Differential Display, mRNA,Differential Displays, mRNA,Expression Analyses, Gene,Expression Analysis, Gene,Gene Expression Analyses,Gene Expression Monitorings,Gene Expression Profilings,Monitoring, Gene Expression,Monitorings, Gene Expression,Profiling, Gene Expression,Profiling, Transcriptome,Profilings, Gene Expression,Profilings, Transcriptome,Transcript Expression Analyses,Transcriptome Analyses,Transcriptome Profilings,mRNA Differential Displays