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We reported the identification, purification and characterization of a low molecular weight protein (Ch 21) expressed in vitro by differentiating chondrocytes at a late stage of development and observed in vivo in the growth plate region of the long bones at the border between hypertrophic cartilage and newly formed bone (Descalzi Cancedda, F., P. Manduca, C. Tacchetti, P. Fossa, R. Quarto, R. Cancedda, J. Cell Biol. 107, 2455-2463 (1988]. In this article, the synthesis and location of Ch 21 protein in the chick embryo tibia at late stage of development were further investigated. Ch 21 was observed in the cartilage matrix surrounding marrow cavities and in the prearticular outer layer by immunolocalization. In addition, the timing of Ch 21 appearance during the tibia development and its distribution in the growth plate region was better defined. We first observed presence of Ch 21 in the perichondral mid-diaphyseal sleeve of 7-day-old tibia. Ch 21 antibodies stained also the newly formed bone. Synthesis and secretion in the culture medium of Ch 21 protein was observed when bone fragments or cultured osteoblasts isolated from 19-day-old embryo tibiae were labeled in vitro. A search for the presence of Ch 21 in the chick embryo sternum was performed. The synthesis of Ch 21, both in the presumptive calcification cranial portion and in the permanent cartilaginous caudal portion of the sternum, was shown by metabolic labeling of tissue slices.(ABSTRACT TRUNCATED AT 250 WORDS)
P Manduca, and
F Descalzi Cancedda, and
C Tacchetti, and
R Quarto, and
P Fossa, and
R Cancedda
May 1990,
Cell and tissue research,
P Manduca, and
F Descalzi Cancedda, and
C Tacchetti, and
R Quarto, and
P Fossa, and
R Cancedda
February 1967,
Journal of molecular biology,
P Manduca, and
F Descalzi Cancedda, and
C Tacchetti, and
R Quarto, and
P Fossa, and
R Cancedda
March 1978,
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P Manduca, and
F Descalzi Cancedda, and
C Tacchetti, and
R Quarto, and
P Fossa, and
R Cancedda
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Journal of embryology and experimental morphology,
P Manduca, and
F Descalzi Cancedda, and
C Tacchetti, and
R Quarto, and
P Fossa, and
R Cancedda
January 1986,
Stain technology,
P Manduca, and
F Descalzi Cancedda, and
C Tacchetti, and
R Quarto, and
P Fossa, and
R Cancedda
September 1971,
Biochimica et biophysica acta,
P Manduca, and
F Descalzi Cancedda, and
C Tacchetti, and
R Quarto, and
P Fossa, and
R Cancedda
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The Journal of biological chemistry,
P Manduca, and
F Descalzi Cancedda, and
C Tacchetti, and
R Quarto, and
P Fossa, and
R Cancedda
November 1993,
The Biochemical journal,
P Manduca, and
F Descalzi Cancedda, and
C Tacchetti, and
R Quarto, and
P Fossa, and
R Cancedda
September 1990,
Journal of cellular biochemistry,
P Manduca, and
F Descalzi Cancedda, and
C Tacchetti, and
R Quarto, and
P Fossa, and
R Cancedda
June 1982,
Experimental eye research,
