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Glycosyl-phosphatidylinositol-specific phospholipase C (GPI-PLC) is a membrane-bound enzyme of bloodstream forms of Trypanosoma brucei, which cleaves the GPI-membrane anchor of the variant surface glycoprotein forming diacylglycerol and 1,2-cyclic phosphate on the inositol ring. The cellular localization of the enzyme was studied by fractionation of sub-cellular organelles and immunofluorescence microscopy and was found to be primarily cytoplasmic. This was confirmed by immuno-electron microscopy using cryo-sections, which showed that the labelling was predominantly on the cytoplasmic side of intracellular membranes but was absent from the plasma membrane including the region lining the flagellar pocket. The significance of these results for the possible function of the phospholipase is discussed.
R Bölow, and
G Griffiths, and
P Webster, and
Y D Stierhof, and
F R Opperdoes, and
P Overath
December 1988,
Proceedings of the National Academy of Sciences of the United States of America,
R Bölow, and
G Griffiths, and
P Webster, and
Y D Stierhof, and
F R Opperdoes, and
P Overath
December 1992,
Molecular and biochemical parasitology,
R Bölow, and
G Griffiths, and
P Webster, and
Y D Stierhof, and
F R Opperdoes, and
P Overath
March 1989,
Molecular and biochemical parasitology,
R Bölow, and
G Griffiths, and
P Webster, and
Y D Stierhof, and
F R Opperdoes, and
P Overath
February 1990,
Molecular and cellular biology,
R Bölow, and
G Griffiths, and
P Webster, and
Y D Stierhof, and
F R Opperdoes, and
P Overath
June 1996,
The Journal of biological chemistry,
R Bölow, and
G Griffiths, and
P Webster, and
Y D Stierhof, and
F R Opperdoes, and
P Overath
November 1986,
The Journal of biological chemistry,
R Bölow, and
G Griffiths, and
P Webster, and
Y D Stierhof, and
F R Opperdoes, and
P Overath
February 1995,
The Journal of biological chemistry,
R Bölow, and
G Griffiths, and
P Webster, and
Y D Stierhof, and
F R Opperdoes, and
P Overath
June 2000,
The Journal of biological chemistry,
R Bölow, and
G Griffiths, and
P Webster, and
Y D Stierhof, and
F R Opperdoes, and
P Overath
November 1991,
Cell biology international reports,
R Bölow, and
G Griffiths, and
P Webster, and
Y D Stierhof, and
F R Opperdoes, and
P Overath
October 1990,
Biochemical Society transactions,
