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We have elaborated three systems of enzyme-linked immunosorbent assay (ELISA) for detection of chicken IgG antibodies specific for hexon antigens of three immunologically distinct adenovirus groups: those of mammalian adenoviruses (Mastadenovira), typical avian adenoviruses (Aviadenovira) and of egg-drop syndrome-76 (EDS-76) virus. In each system the antibodies against respective hexons were specifically detected. In mammalian adenovirus hexons the ELISA detects primarily the type-specific (epsilon) and genus-specific (alpha) antigenic determinants. The time course of anti-hexon antibodies content was followed during immunization. The level of anti-hexon antibodies in egg yolk reflects adequately their content in blood serum. The technique is suitable for serological diagnosis of chicken adenoviral infections as well as for characterization of egg-yolk antibodies obtained by preparative hyperimmunization of hens.
S N Khil'ko, and
M A Kirasova, and
S D Piker, and
S D Osidze, and
N V Fomina, and
M P Burgasova, and
T I Tikhonenko
January 2000,
Molekuliarnaia genetika, mikrobiologiia i virusologiia,
S N Khil'ko, and
M A Kirasova, and
S D Piker, and
S D Osidze, and
N V Fomina, and
M P Burgasova, and
T I Tikhonenko
January 1987,
Archives of virology,
S N Khil'ko, and
M A Kirasova, and
S D Piker, and
S D Osidze, and
N V Fomina, and
M P Burgasova, and
T I Tikhonenko
January 1987,
Intervirology,
S N Khil'ko, and
M A Kirasova, and
S D Piker, and
S D Osidze, and
N V Fomina, and
M P Burgasova, and
T I Tikhonenko
January 1984,
Acta microbiologica Hungarica,
S N Khil'ko, and
M A Kirasova, and
S D Piker, and
S D Osidze, and
N V Fomina, and
M P Burgasova, and
T I Tikhonenko
June 2023,
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
S N Khil'ko, and
M A Kirasova, and
S D Piker, and
S D Osidze, and
N V Fomina, and
M P Burgasova, and
T I Tikhonenko
July 2022,
Viruses,
S N Khil'ko, and
M A Kirasova, and
S D Piker, and
S D Osidze, and
N V Fomina, and
M P Burgasova, and
T I Tikhonenko
September 1988,
Acta virologica,
S N Khil'ko, and
M A Kirasova, and
S D Piker, and
S D Osidze, and
N V Fomina, and
M P Burgasova, and
T I Tikhonenko
January 1982,
Voprosy virusologii,
S N Khil'ko, and
M A Kirasova, and
S D Piker, and
S D Osidze, and
N V Fomina, and
M P Burgasova, and
T I Tikhonenko
January 1984,
Voprosy meditsinskoi khimii,
S N Khil'ko, and
M A Kirasova, and
S D Piker, and
S D Osidze, and
N V Fomina, and
M P Burgasova, and
T I Tikhonenko
September 1998,
Nippon Ganka Gakkai zasshi,
