BIOMAT Database Logo BIOMAT Database Logo

Prevalence and Level of Enterohemorrhagic Escherichia coli in Culled Dairy Cows at Harvest. 2016

Zachary R Stromberg, and Gentry L Lewis, and Sharif S Aly, and Terry W Lehenbauer, and Joseph M Bosilevac, and Natalia Cernicchiaro, and Rodney A Moxley
School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583, USA.

The primary objective of this study was to determine the prevalence and level of enterohemorrhagic Escherichia coli (EHEC) O26, O45, O103, O111, O121, and O145 (collectively EHEC-6) plus EHEC O157 in fecal, hide, and preintervention carcass surface samples from culled dairy cows. Matched samples (n = 300) were collected from 100 cows at harvest and tested by a culture-based method and two molecular methods: NeoSEEK STEC (NS) and Atlas STEC EG2 Combo. Both the culture and NS methods can be used to discriminate among the seven EHEC types (EHEC-7), from which the cumulative prevalence was inferred, whereas the Atlas method can discriminate only between EHEC O157 and non-O157 EHEC, without discrimination of the serogroup. The EHEC-7 prevalence in feces, hides, and carcass surfaces was 6.5, 15.6, and 1.0%, respectively, with the culture method and 25.9, 64.9, and 7.0%, respectively, with the NS method. With the Atlas method, the prevalence of non-O157 EHEC was 29.1, 38.3, and 28.0% and that of EHEC O157 was 29.1, 57.0, and 3.0% for feces, hides, and carcasses, respectively. Only two samples (a hide sample and a fecal sample) originating from different cows contained quantifiable EHEC. In both samples, the isolates were identified as EHEC O157, with 4.7 CFU/1,000 cm(2) in the hide sample and 3.9 log CFU/g in the fecal sample. Moderate agreement was found between culture and NS results for detection of EHEC O26 (κ = 0.58, P < 0.001), EHEC O121 (κ = 0.50, P < 0.001), and EHEC O157 (κ = 0.40, P < 0.001). No significant agreement was observed between NS and Atlas results or between culture and Atlas results. Detection of an EHEC serogroup in fecal samples was significantly associated with detection of the same EHEC serogroup in hide samples for EHEC O26 (P = 0.001), EHEC O111 (P = 0.002), EHEC O121 (P < 0.001), and EHEC-6 (P = 0.029) based on NS detection and for EHEC O121 (P < 0.001) based on detection by culture. This study provides evidence that non-O157 EHEC are ubiquitous on hides of culled dairy cattle and that feces are an important source of non-O157 EHEC hide contamination.

UI MeSH Term Description Entries
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D003430 Cross-Sectional Studies Studies in which the presence or absence of disease or other health-related variables are determined in each member of the study population or in a representative sample at one particular time. This contrasts with LONGITUDINAL STUDIES which are followed over a period of time. Disease Frequency Surveys,Prevalence Studies,Analysis, Cross-Sectional,Cross Sectional Analysis,Cross-Sectional Survey,Surveys, Disease Frequency,Analyses, Cross Sectional,Analyses, Cross-Sectional,Analysis, Cross Sectional,Cross Sectional Analyses,Cross Sectional Studies,Cross Sectional Survey,Cross-Sectional Analyses,Cross-Sectional Analysis,Cross-Sectional Study,Cross-Sectional Surveys,Disease Frequency Survey,Prevalence Study,Studies, Cross-Sectional,Studies, Prevalence,Study, Cross-Sectional,Study, Prevalence,Survey, Cross-Sectional,Survey, Disease Frequency,Surveys, Cross-Sectional
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D005243 Feces Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D054323 Shiga-Toxigenic Escherichia coli Strains of ESCHERICHIA COLI with the ability to produce at least one or more of at least two antigenically distinct, usually bacteriophage-mediated cytotoxins: SHIGA TOXIN 1 and SHIGA TOXIN 2. These bacteria can cause severe disease in humans including bloody DIARRHEA and HEMOLYTIC UREMIC SYNDROME. E coli, Verotoxigenic,Escherichia coli, Verotoxigenic,STEC,Verotoxigenic Escherichia coli,Shiga Toxigenic E. coli,Shiga Toxigenic Escherichia coli,Shiga Toxin-Producing Escherichia coli,VTEC,Vero Cytotoxin-Producing Escherichia coli,Verotoxigenic E. coli,Verotoxin-Producing Escherichia coli
D054324 Enterohemorrhagic Escherichia coli Strains of ESCHERICHIA COLI that are a subgroup of SHIGA-TOXIGENIC ESCHERICHIA COLI. They cause non-bloody and bloody DIARRHEA; HEMOLYTIC UREMIC SYNDROME; and hemorrhagic COLITIS. An important member of this subgroup is ESCHERICHIA COLI O157-H7. E coli, Enterohemorrhagic,EHEC,Escherichia coli, Enterohemorrhagic,Enterohemorrhagic E coli,Enterohemorrhagic E. coli
D019453 Escherichia coli O157 A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. A strain from this serogroup, serotype H7, which produces SHIGA TOXINS, has been linked to human disease outbreaks resulting from contamination of foods by E. coli O157 from bovine origin. E coli O157,E coli O157-H7,Escherichia coli O157-H7

Related Publications

Zachary R Stromberg, and Gentry L Lewis, and Sharif S Aly, and Terry W Lehenbauer, and Joseph M Bosilevac, and Natalia Cernicchiaro, and Rodney A Moxley
Cow-level prevalence of paratuberculosis in culled dairy cows in Atlantic Canada and Maine.
November 2004, Journal of dairy science,
Zachary R Stromberg, and Gentry L Lewis, and Sharif S Aly, and Terry W Lehenbauer, and Joseph M Bosilevac, and Natalia Cernicchiaro, and Rodney A Moxley
Prevalence of enterohemorrhagic Escherichia coli O157:H7 in a survey of dairy herds.
April 1995, Applied and environmental microbiology,
Zachary R Stromberg, and Gentry L Lewis, and Sharif S Aly, and Terry W Lehenbauer, and Joseph M Bosilevac, and Natalia Cernicchiaro, and Rodney A Moxley
Verotoxin-producing Escherichia coli in culled beef cows grazing rangeland forages.
April 2003, Experimental biology and medicine (Maywood, N.J.),
Zachary R Stromberg, and Gentry L Lewis, and Sharif S Aly, and Terry W Lehenbauer, and Joseph M Bosilevac, and Natalia Cernicchiaro, and Rodney A Moxley
Pre-Harvest Survival and Post-Harvest Chlorine Tolerance of Enterohemorrhagic Escherichia coli on Lettuce.
November 2019, Toxins,
Zachary R Stromberg, and Gentry L Lewis, and Sharif S Aly, and Terry W Lehenbauer, and Joseph M Bosilevac, and Natalia Cernicchiaro, and Rodney A Moxley
Proportions and Serogroups of Enterohemorrhagic Shiga Toxin-producing Escherichia coli in Feces of Fed and Cull Beef and Cull Dairy Cattle at Harvest.
April 2024, Journal of food protection,
Zachary R Stromberg, and Gentry L Lewis, and Sharif S Aly, and Terry W Lehenbauer, and Joseph M Bosilevac, and Natalia Cernicchiaro, and Rodney A Moxley
Escherichia coli O157 in Cull Dairy Cows on Farm and at Slaughter.
November 1997, Journal of food protection,
Zachary R Stromberg, and Gentry L Lewis, and Sharif S Aly, and Terry W Lehenbauer, and Joseph M Bosilevac, and Natalia Cernicchiaro, and Rodney A Moxley
Enterohemorrhagic Escherichia coli.
January 2001, Contributions to microbiology,
Zachary R Stromberg, and Gentry L Lewis, and Sharif S Aly, and Terry W Lehenbauer, and Joseph M Bosilevac, and Natalia Cernicchiaro, and Rodney A Moxley
Enterohemorrhagic Escherichia coli.
February 1998, Current opinion in microbiology,
Zachary R Stromberg, and Gentry L Lewis, and Sharif S Aly, and Terry W Lehenbauer, and Joseph M Bosilevac, and Natalia Cernicchiaro, and Rodney A Moxley
Enterohemorrhagic Escherichia coli.
December 1987, The New England journal of medicine,
Zachary R Stromberg, and Gentry L Lewis, and Sharif S Aly, and Terry W Lehenbauer, and Joseph M Bosilevac, and Natalia Cernicchiaro, and Rodney A Moxley
Escherichia coli O157:H7 in healthy dairy cows.
April 1997, New Zealand veterinary journal,
Copied contents to your clipboard!