Mycoplasma hyopneumoniae genetic variability within a swine operation. 2016

Lucina Galina Pantoja, and Kalie Pettit, and Lucas F Dos Santos, and Rick Tubbs, and Maria Pieters
Zoetis, Florham Park, NJ (Galina Pantoja)University of Minnesota, St. Paul, MN (Pettit, Dos Santos, Pieters)Tubbs Contract Research Organization, Jasper, AL (Tubbs) lucina.galina@zoetis.com.

The objective of our study was to characterize the Mycoplasma hyopneumoniae genetic diversity within a swine operation comingling weaned pigs. Bronchial swabs and tracheal aspirates were collected from 3 nursery-to-finish farms. During the finishing production stages, samples were obtained from mortalities and from live coughing pigs in rooms where mortality was not observed. A total of 105 samples were examined by a M. hyopneumoniae real-time polymerase chain reaction and subjected to genetic typing using a multilocus variable number tandem repeat analysis (MLVA) assay. The MLVA was used to identify genetic variants based on the number of repeats in 2 variable number tandem repeats loci, namely P97 and P146, thought to mediate adherence of M. hyopneumoniae to swine cilia. Four distinguishable M. hyopneumoniae variants were identified: MVLA variants 9-15, 11-21, 9-21, and 7-15. Variant 9-15 was the most prevalent, observed in 79% of rooms, and detected on all 3 farms. Variant 11-21 was present in 37% of the rooms on 2 of the 3 farms. Only one 9-21 variant was identified in 1 farm, and all samples of variant 7-15 were recovered from another farm. Based on the low prevalence and limited geographic distribution of the last 2 variants, it is hypothesized that they might be the result of in-situ recombination. All variants detected in this investigation appeared to belong to 3 clusters. Overall, a limited number of variants and clusters were identified in a system that comingles pigs from different sources, suggesting limited M. hyopneumoniae genetic variation within commercial swine production environments.

UI MeSH Term Description Entries
D001938 Brazil A country located on the eastern coast of South America, located between Colombia and Peru, that borders the Atlantic Ocean. It is bordered on the north by Venezuela, Guyana, Suriname, and French Guiana, on the south by Uruguay, and on the west by Argentina. The capital is Brasilia.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D014644 Genetic Variation Genotypic differences observed among individuals in a population. Genetic Diversity,Variation, Genetic,Diversity, Genetic,Diversities, Genetic,Genetic Diversities,Genetic Variations,Variations, Genetic
D014886 Weaning Permanent deprivation of breast milk and commencement of nourishment with other food. (From Stedman, 25th ed) Weanings
D045705 Mycoplasma hyopneumoniae A species of gram-negative bacteria that causes MYCOPLASMA PNEUMONIA OF SWINE. The organism damages the CILIA in the airways of the pig, and thus compromises one of the most effective mechanical barriers against invading pathogens. The resulting weakening of the IMMUNE SYSTEM can encourage secondary infections, leading to porcine respiratory disease complex.
D045729 Pneumonia of Swine, Mycoplasmal A chronic, clinically mild, infectious pneumonia of PIGS caused by MYCOPLASMA HYOPNEUMONIAE. Ninety percent of swine herds worldwide are infected with this economically costly disease that primarily affects animals aged two to six months old. The disease can be associated with porcine respiratory disease complex. PASTEURELLA MULTOCIDA is often found as a secondary infection. Enzootic Pneumonia of Swine,Mycoplasmal Pneumonia of Swine,Pneumonia of Swine, Enzootic,Enzootic Pneumonia of Pigs,Mycoplasma Pneumonia of Pigs,Mycoplasma Pneumonia of Swine,Swine Enzootic Pneumonia,Swine Mycoplasma Pneumonia,Swine Mycoplasmal Pneumonia
D060888 Real-Time Polymerase Chain Reaction Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction. Kinetic Polymerase Chain Reaction,Quantitative Real-Time PCR,Quantitative Real-Time Polymerase Chain Reaction,Real-Time PCR,PCR, Quantitative Real-Time,PCR, Real-Time,PCRs, Quantitative Real-Time,PCRs, Real-Time,Quantitative Real Time PCR,Quantitative Real Time Polymerase Chain Reaction,Quantitative Real-Time PCRs,Real Time PCR,Real Time Polymerase Chain Reaction,Real-Time PCR, Quantitative,Real-Time PCRs,Real-Time PCRs, Quantitative
D018598 Minisatellite Repeats Tandem arrays of moderately repetitive, short (10-60 bases) DNA sequences which are found dispersed throughout the GENOME, at the ends of chromosomes (TELOMERES), and clustered near telomeres. Their degree of repetition is two to several hundred at each locus. Loci number in the thousands but each locus shows a distinctive repeat unit. Minisatellite,Minisatellite Repeat,VNTR Loci,VNTR Region,VNTR Sequence,VNTR Sequences,Variable Number Of Tandem Repeat,Variable Number Tandem Repeat,Variable Number of Tandem Repeats,Variable Tandem Repeat,Minisatellites,Variable Number Tandem Repeats,Variable Tandem Repeats,Loci, VNTR,Locus, VNTR,Region, VNTR,Regions, VNTR,Repeat, Minisatellite,Repeat, Variable Tandem,Repeats, Minisatellite,Repeats, Variable Tandem,Sequence, VNTR,Sequences, VNTR,Tandem Repeat, Variable,Tandem Repeats, Variable,VNTR Locus,VNTR Regions

Related Publications

Lucina Galina Pantoja, and Kalie Pettit, and Lucas F Dos Santos, and Rick Tubbs, and Maria Pieters
October 2020, Canadian journal of veterinary research = Revue canadienne de recherche veterinaire,
Lucina Galina Pantoja, and Kalie Pettit, and Lucas F Dos Santos, and Rick Tubbs, and Maria Pieters
January 2021, Porcine health management,
Lucina Galina Pantoja, and Kalie Pettit, and Lucas F Dos Santos, and Rick Tubbs, and Maria Pieters
March 2007, Veterinary microbiology,
Lucina Galina Pantoja, and Kalie Pettit, and Lucas F Dos Santos, and Rick Tubbs, and Maria Pieters
March 1973, The Journal of infectious diseases,
Lucina Galina Pantoja, and Kalie Pettit, and Lucas F Dos Santos, and Rick Tubbs, and Maria Pieters
September 2017, Veterinary microbiology,
Lucina Galina Pantoja, and Kalie Pettit, and Lucas F Dos Santos, and Rick Tubbs, and Maria Pieters
July 2001, Journal of clinical microbiology,
Lucina Galina Pantoja, and Kalie Pettit, and Lucas F Dos Santos, and Rick Tubbs, and Maria Pieters
April 1994, The Journal of veterinary medical science,
Lucina Galina Pantoja, and Kalie Pettit, and Lucas F Dos Santos, and Rick Tubbs, and Maria Pieters
January 1995, Veterinary research,
Lucina Galina Pantoja, and Kalie Pettit, and Lucas F Dos Santos, and Rick Tubbs, and Maria Pieters
January 1970, National Institute of Animal Health quarterly,
Lucina Galina Pantoja, and Kalie Pettit, and Lucas F Dos Santos, and Rick Tubbs, and Maria Pieters
October 1983, American journal of veterinary research,
Copied contents to your clipboard!