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The enzyme-linked immunoassay modification has been worked out. The method combines advantages of membrane technology of antigen immobilization which is used in the enzyme immunosensory technique and of conventional enzyme-linked immunosorbent assay. The nitrocellulose and polypropylene membranes are used as a solid-phase. The purified rabbit immunoglobulin G is immobilized on the surface of membranes as the first layer. The competitive immunoassay is employed. The immunoglobulin G concentration range is 1-1000 ng/ml. The membranes with the immobilized antigen can be repeatedly used after incubation in 0.1 M glycine buffer, pH 2.5. The dry membrane with the immobilized antigen can be used after keeping for 6 months in refrigerator at 4 degrees C without changing the concentration range measured.
P Ia Arenkov, and
E N Zhmareva, and
V A Berezin
January 1989,
Laboratornoe delo,
P Ia Arenkov, and
E N Zhmareva, and
V A Berezin
March 1984,
Archiv fur experimentelle Veterinarmedizin,
P Ia Arenkov, and
E N Zhmareva, and
V A Berezin
January 1991,
Laboratornoe delo,
P Ia Arenkov, and
E N Zhmareva, and
V A Berezin
May 1984,
Zhurnal mikrobiologii, epidemiologii i immunobiologii,
P Ia Arenkov, and
E N Zhmareva, and
V A Berezin
January 1988,
Trudy Instituta imeni Pastera,
P Ia Arenkov, and
E N Zhmareva, and
V A Berezin
January 1991,
Voprosy virusologii,
P Ia Arenkov, and
E N Zhmareva, and
V A Berezin
January 1990,
Laboratornoe delo,
P Ia Arenkov, and
E N Zhmareva, and
V A Berezin
February 1990,
Enzyme and microbial technology,
P Ia Arenkov, and
E N Zhmareva, and
V A Berezin
January 1993,
Sudebno-meditsinskaia ekspertiza,
P Ia Arenkov, and
E N Zhmareva, and
V A Berezin
January 1986,
Voprosy virusologii,
