-transparent.png){kind=logo}
Genes encoding sequence-specific DNA binding proteins can be isolated by screening lambda gt11 expression libraries with recognition site DNAs. This strategy is derived from that developed for the isolation of genes using antibody probes. Many different genes encoding transcriptional regulatory proteins have been cloned using this strategy. The DNA binding domains of these regulatory proteins contain different structural motifs including the helix-turn-helix, the "zinc finger" and the "leucine zipper". Various aspects of the screening strategy are evaluated and a detailed protocol is provided. In addition to binding site DNAs, protein and nucleotide probes have been successfully used to screen expression libraries. Therefore ligand based expression screening may be quite general in scope.
H Singh, and
R G Clerc, and
J H LeBowitz
January 1997,
Methods in molecular biology (Clifton, N.J.),
H Singh, and
R G Clerc, and
J H LeBowitz
January 1993,
Methods in enzymology,
H Singh, and
R G Clerc, and
J H LeBowitz
January 1994,
Methods in molecular biology (Clifton, N.J.),
H Singh, and
R G Clerc, and
J H LeBowitz
October 1990,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
H Singh, and
R G Clerc, and
J H LeBowitz
January 2016,
PloS one,
H Singh, and
R G Clerc, and
J H LeBowitz
January 1991,
Analytical biochemistry,
H Singh, and
R G Clerc, and
J H LeBowitz
January 2004,
Methods in cell biology,
H Singh, and
R G Clerc, and
J H LeBowitz
May 1992,
Proceedings of the National Academy of Sciences of the United States of America,
H Singh, and
R G Clerc, and
J H LeBowitz
January 2003,
Gene,
H Singh, and
R G Clerc, and
J H LeBowitz
May 1991,
Proceedings of the National Academy of Sciences of the United States of America,
