Improved ethanol production in the presence of cadmium ions by a Saccharomyces cerevisiae transformed with a novel cadmium-resistance gene DvCRP1. 2016

Jiajun Hu, and Qingyun Xu, and Mengnan Wu, and Xiangzong Meng, and Rentao Song, and Mintian Gao
a Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences , Shanghai University , Shanghai , People's Republic of China.

The DvCRP1 gene obtained from Dunaliella viridis is a cadmium-resistance gene that induces cadmium accumulation in microbial and plant cells. In the present study, Saccharomyces cerevisiae was used as a model system to investigate the effect of DvCRP1 on both cadmium detoxification and ethanol production. Inhibitory effects of cadmium (50-300 µmol/L) on growth (29-92%), glucose consumption (23-89%), and ethanol production (17-92%) were observed at 24 h by S. cerevisiae. DvCRP1 alleviated the inhibitory effect of cadmium, with increase in the ethanol production. The established mathematical model showed that the initial inoculation concentration, cadmium concentration, and transformation of DvCRP1 were the most important factors for cell growth, glucose consumption, and ethanol production. Cadmium detoxification of yeast was also enhanced by increasing the initial concentration of yeast cells. Transforming with DvCRP1 further enhanced detoxification, especially at high cadmium concentrations. Transforming with DvCRP1 further enhanced detoxification, especially at high cadmium concentrations (200 µmol/L). The present results evidenced the potential of the insertion of the DvCRP1 gene into yeast for use in bio-refineries during fermentation of heavy metals-contaminated substrates. In addition, this is a promising method for phytoremediation of agricultural soils highly contaminated by heavy metals.

UI MeSH Term Description Entries
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D002104 Cadmium An element with atomic symbol Cd, atomic number 48, and atomic weight 112.41. It is a metal and ingestion will lead to CADMIUM POISONING.
D004351 Drug Resistance Diminished or failed response of an organism, disease or tissue to the intended effectiveness of a chemical or drug. It should be differentiated from DRUG TOLERANCE which is the progressive diminution of the susceptibility of a human or animal to the effects of a drug, as a result of continued administration. Resistance, Drug
D005947 Glucose A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement. Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D005978 Glutathione A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides. Reduced Glutathione,gamma-L-Glu-L-Cys-Gly,gamma-L-Glutamyl-L-Cysteinylglycine,Glutathione, Reduced,gamma L Glu L Cys Gly,gamma L Glutamyl L Cysteinylglycine
D000431 Ethanol A clear, colorless liquid rapidly absorbed from the gastrointestinal tract and distributed throughout the body. It has bactericidal activity and is used often as a topical disinfectant. It is widely used as a solvent and preservative in pharmaceutical preparations as well as serving as the primary ingredient in ALCOHOLIC BEVERAGES. Alcohol, Ethyl,Absolute Alcohol,Grain Alcohol,Alcohol, Absolute,Alcohol, Grain,Ethyl Alcohol
D000460 Chlorophyta A phylum of photosynthetic EUKARYOTA bearing double membrane-bound plastids containing chlorophyll a and b. They comprise the classical green algae, and represent over 7000 species that live in a variety of primarily aquatic habitats. Only about ten percent are marine species, most live in freshwater. Algae, Green,Chlorophytina,Green Algae
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D020418 Algal Proteins Proteins found in any species of algae. Algal Gene Product,Algal Gene Products,Algal Gene Protein,Algal Gene Proteins,Algal Peptide,Algal Peptides,Algal Protein,Gene Products, Algal,Gene Product, Algal,Gene Protein, Algal,Gene Proteins, Algal,Peptide, Algal,Peptides, Algal,Product, Algal Gene,Protein, Algal,Protein, Algal Gene,Proteins, Algal

Related Publications

Jiajun Hu, and Qingyun Xu, and Mengnan Wu, and Xiangzong Meng, and Rentao Song, and Mintian Gao
February 2010, Applied biochemistry and biotechnology,
Jiajun Hu, and Qingyun Xu, and Mengnan Wu, and Xiangzong Meng, and Rentao Song, and Mintian Gao
April 1992, Biotechnology and bioengineering,
Jiajun Hu, and Qingyun Xu, and Mengnan Wu, and Xiangzong Meng, and Rentao Song, and Mintian Gao
March 2015, Journal of bioscience and bioengineering,
Jiajun Hu, and Qingyun Xu, and Mengnan Wu, and Xiangzong Meng, and Rentao Song, and Mintian Gao
June 2016, Journal of bioscience and bioengineering,
Jiajun Hu, and Qingyun Xu, and Mengnan Wu, and Xiangzong Meng, and Rentao Song, and Mintian Gao
October 1989, Molecular & general genetics : MGG,
Jiajun Hu, and Qingyun Xu, and Mengnan Wu, and Xiangzong Meng, and Rentao Song, and Mintian Gao
October 1998, Applied microbiology and biotechnology,
Jiajun Hu, and Qingyun Xu, and Mengnan Wu, and Xiangzong Meng, and Rentao Song, and Mintian Gao
October 1997, Journal of industrial microbiology & biotechnology,
Jiajun Hu, and Qingyun Xu, and Mengnan Wu, and Xiangzong Meng, and Rentao Song, and Mintian Gao
December 2006, Biotechnology letters,
Jiajun Hu, and Qingyun Xu, and Mengnan Wu, and Xiangzong Meng, and Rentao Song, and Mintian Gao
January 2019, 3 Biotech,
Jiajun Hu, and Qingyun Xu, and Mengnan Wu, and Xiangzong Meng, and Rentao Song, and Mintian Gao
January 2013, SpringerPlus,
Copied contents to your clipboard!