Biomaterial Database

The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. 2016

ZhenChao Zhang, and XinChao Liu, and XinChao Yang, and LianRui Liu, and Shuai Wang, and MingMin Lu, and Mohammad Ehsan, and Javaid A Gadahi, and XiaoKai Song, and LiXin Xu, and RuoFeng Yan, and XiangRui Li

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.

The gene of Eimeria acervulina microneme protein 3 (EaMIC3) was cloned and characterized. According to the conserved sequence, the 3'- and 5'-ends of EaMIC3 were amplified by the rapid amplification of cDNA ends (RACE). The full length cDNA of this gene was obtained by overlapping the sequences of 3'- and 5'-extremities and amplification by reverse transcription PCR. The sequence analysis revealed that the opening reading frame (ORF) of EaMIC3 was 2,607 bp and encoded a protein of 868 amino acids with 93.04 kDa. Western blotting assay showed that the recombinant protein was successfully recognized by the sera of chickens experimentally infected with E. acervulina, whereas the native protein in the somatic extract of sporozoites was as well detected by sera from rats immunized with the recombinant protein of EaMIC3. Immunofluorescence analysis indicated that EaMIC3 was expressed in the sporozoites and merozoites stages of E. acervulina. Animal challenge experiments demonstrated that the recombinant protein of EaMIC3 could significantly increase the average body weight gains, decrease the mean lesion scores and the oocyst outputs of the immunized chickens and presented anticoccidial index (ACI) more than 165. Moreover, EaMIC3 protein produced significantly high level of IgG antibody, IFN-γ, IL-10, IL-17 TGF-β, CD4+ , and CD8+ .

UI	MeSH Term	Description	Entries
D011201	Poultry Diseases	Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild.	Disease, Poultry,Diseases, Poultry,Poultry Disease
D002645	Chickens	Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.	Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D003048	Coccidiosis	Protozoan infection found in animals and man. It is caused by several different genera of COCCIDIA.	Besnoitiasis,Besnoitiosis,Besnoitiases,Besnoitioses,Coccidioses
D004539	Eimeria	A genus of protozoan parasites of the subclass COCCIDIA. Various species are parasitic in the epithelial cells of the liver and intestines of man and other animals.	Eimerias
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000913	Antibodies, Protozoan	Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.	Protozoan Antibodies
D015800	Protozoan Proteins	Proteins found in any species of protozoan.	Proteins, Protozoan
D016753	Interleukin-10	A cytokine produced by a variety of cell types, including T-LYMPHOCYTES; MONOCYTES; DENDRITIC CELLS; and EPITHELIAL CELLS that exerts a variety of effects on immunoregulation and INFLAMMATION. Interleukin-10 combines with itself to form a homodimeric molecule that is the biologically active form of the protein.	IL-10,CSIF-10,Cytokine Synthesis Inhibitory Factor,IL10,Interleukin 10
D020381	Interleukin-17	A proinflammatory cytokine produced primarily by T-LYMPHOCYTES or their precursors. Several subtypes of interleukin-17 have been identified, each of which is a product of a unique gene.	IL-17,CTLA-8,CTLA8,Cytokine CX2,Cytokine ML-1,Cytotoxic T lymphocyte-Associated Antigen 8,IL-17A,IL-17B,IL-17C,IL-17E,IL-17F,Interleukin 17,Interleukin-17A,Interleukin-17B,Interleukin-17C,Interleukin-17E,Interleukin-17F,Interleukin-25,CX2, Cytokine,Cytokine ML 1,Cytotoxic T lymphocyte Associated Antigen 8,IL 17E,Interleukin 17A,Interleukin 17B,Interleukin 17C,Interleukin 17E,Interleukin 17F,Interleukin 25