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The sequence of the 841 amino acid residues in each subunit (molecular weight 97,412) of rabbit muscle glycogen phosphorylase b (1,4-alpha-D-glucan:orthophosphate alpha-glucosyltransferase; EC 2.4.1.1) has been determined. The general strategy was based on limited proteolysis of native phosphorylase b by subtilisin BPN', yielding two large segments (light and heavy) which were fragmented by cleavage at methyonyl-, asparaginyl-glycine, and aspartyl-proline bonds. Analysis of two cyanogen bromide fragments (CB14 and CB17) isolated from the intact molecule yielded the overlap between the light and heavy fragments and the remainder of the sequence. The residues involved in the covalent and allosteric control of the enzyme, and in the binding of the cofactor pyridoxal 5'-phosphate, were identified as serine-14, tyrosine-155, and lysine-679, respectively.
K Titani, and
A Koide, and
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S Kumar, and
R D Wade, and
K A Walsh, and
H Neurath, and
E H Fischer
August 1986,
FEBS letters,
K Titani, and
A Koide, and
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L H Ericsson, and
S Kumar, and
R D Wade, and
K A Walsh, and
H Neurath, and
E H Fischer
April 1970,
Biochemistry,
K Titani, and
A Koide, and
J Hermann, and
L H Ericsson, and
S Kumar, and
R D Wade, and
K A Walsh, and
H Neurath, and
E H Fischer
July 1975,
FEBS letters,
K Titani, and
A Koide, and
J Hermann, and
L H Ericsson, and
S Kumar, and
R D Wade, and
K A Walsh, and
H Neurath, and
E H Fischer
August 1983,
The Journal of biological chemistry,
K Titani, and
A Koide, and
J Hermann, and
L H Ericsson, and
S Kumar, and
R D Wade, and
K A Walsh, and
H Neurath, and
E H Fischer
October 1985,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
K Titani, and
A Koide, and
J Hermann, and
L H Ericsson, and
S Kumar, and
R D Wade, and
K A Walsh, and
H Neurath, and
E H Fischer
September 1973,
Proceedings of the National Academy of Sciences of the United States of America,
K Titani, and
A Koide, and
J Hermann, and
L H Ericsson, and
S Kumar, and
R D Wade, and
K A Walsh, and
H Neurath, and
E H Fischer
April 1963,
The Journal of biological chemistry,
K Titani, and
A Koide, and
J Hermann, and
L H Ericsson, and
S Kumar, and
R D Wade, and
K A Walsh, and
H Neurath, and
E H Fischer
December 1978,
Biochemistry,
K Titani, and
A Koide, and
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L H Ericsson, and
S Kumar, and
R D Wade, and
K A Walsh, and
H Neurath, and
E H Fischer
October 1985,
European journal of biochemistry,
K Titani, and
A Koide, and
J Hermann, and
L H Ericsson, and
S Kumar, and
R D Wade, and
K A Walsh, and
H Neurath, and
E H Fischer
May 1975,
Biochemistry,
