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The action of elastase on elastin was measured by high-performance liquid chromatography with on-line post-column derivatization. After alkaline hydrolysis, a 20-ml sample was injected into a ODS-2 gel column. An elastin-specific dipeptide, Val-Pro, and the internal standard, Gly-Leu, were eluted by a linear gradient of 0 to 10% of 1-propanol in 50 mM heptafluorobutyrate (pH 3) at a flow-rate of 1 ml/min. The eluent was reacted with fluorescamine, and the fluorescent products were measured. Retention times for Val-Pro and Gly-Leu were 17.33 and 23.54 min. The peak areas of 0.2-16 micrograms of Val-Pro gave a straight-line plot. Elastase activity was constant from 6 to 24 h and was 0.95 +/- 0.02 (S.D.) micrograms/h. The method may be useful for the measurement of the elastolytic activity in some diseased tissues.
T A Stein, and
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L Wise
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Se pu = Chinese journal of chromatography,
T A Stein, and
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L Wise
May 2000,
Se pu = Chinese journal of chromatography,
T A Stein, and
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T A Stein, and
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T A Stein, and
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T A Stein, and
J R Cohen, and
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L Wise
December 2013,
SpringerPlus,
T A Stein, and
J R Cohen, and
C Mandell, and
L Wise
March 2000,
Se pu = Chinese journal of chromatography,
T A Stein, and
J R Cohen, and
C Mandell, and
L Wise
January 2002,
Se pu = Chinese journal of chromatography,
T A Stein, and
J R Cohen, and
C Mandell, and
L Wise
September 2005,
Se pu = Chinese journal of chromatography,
T A Stein, and
J R Cohen, and
C Mandell, and
L Wise
January 2004,
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