-transparent.png){kind=logo}
Adaptation of the [3H]Hypoxanthine Uptake Assay for In Vitro-Cultured Plasmodium knowlesi Malaria Parasites. 2016
Megan S J Arnold, and
Jessica A Engel, and
Ming Jang Chua, and
Gillian M Fisher, and
Tina S Skinner-Adams, and
Katherine T Andrews
Eskitis Institute for Drug Discovery, Griffith University, Queensland, Australia.
The zoonotic malaria parasite Plasmodium knowlesi has recently been established in continuous in vitro culture. Here, the Plasmodium falciparum [(3)H]hypoxanthine uptake assay was adapted for P. knowlesi and used to determine the sensitivity of this parasite to chloroquine, cycloguanil, and clindamycin. The data demonstrate that P. knowlesi is sensitive to all drugs, with 50% inhibitory concentrations (IC50s) consistent with those obtained with P. falciparum This assay provides a platform to use P. knowlesi in vitro for drug discovery.
Related Publications
Megan S J Arnold, and
Jessica A Engel, and
Ming Jang Chua, and
Gillian M Fisher, and
Tina S Skinner-Adams, and
Katherine T Andrews
January 2023,
Frontiers in veterinary science,
Megan S J Arnold, and
Jessica A Engel, and
Ming Jang Chua, and
Gillian M Fisher, and
Tina S Skinner-Adams, and
Katherine T Andrews
March 1983,
The Journal of clinical investigation,
Megan S J Arnold, and
Jessica A Engel, and
Ming Jang Chua, and
Gillian M Fisher, and
Tina S Skinner-Adams, and
Katherine T Andrews
September 2010,
Emerging infectious diseases,
Megan S J Arnold, and
Jessica A Engel, and
Ming Jang Chua, and
Gillian M Fisher, and
Tina S Skinner-Adams, and
Katherine T Andrews
February 2019,
The Journal of infectious diseases,
Megan S J Arnold, and
Jessica A Engel, and
Ming Jang Chua, and
Gillian M Fisher, and
Tina S Skinner-Adams, and
Katherine T Andrews
June 2022,
PLoS pathogens,
Megan S J Arnold, and
Jessica A Engel, and
Ming Jang Chua, and
Gillian M Fisher, and
Tina S Skinner-Adams, and
Katherine T Andrews
April 2019,
Malaria journal,
Megan S J Arnold, and
Jessica A Engel, and
Ming Jang Chua, and
Gillian M Fisher, and
Tina S Skinner-Adams, and
Katherine T Andrews
October 2015,
Proceedings of the National Academy of Sciences of the United States of America,
Megan S J Arnold, and
Jessica A Engel, and
Ming Jang Chua, and
Gillian M Fisher, and
Tina S Skinner-Adams, and
Katherine T Andrews
September 2010,
The Medical journal of Malaysia,
Megan S J Arnold, and
Jessica A Engel, and
Ming Jang Chua, and
Gillian M Fisher, and
Tina S Skinner-Adams, and
Katherine T Andrews
May 2011,
Emerging infectious diseases,
Megan S J Arnold, and
Jessica A Engel, and
Ming Jang Chua, and
Gillian M Fisher, and
Tina S Skinner-Adams, and
Katherine T Andrews
May 1945,
Science (New York, N.Y.),
