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Acetoacetyl-CoA synthetase (AACS) is a ketone body-utilizing enzyme and is responsible for the synthesis of cholesterol and fatty acids. We have previously shown that AACS is cleaved by legumain, a lysosomal asparaginyl endopeptidase. In this study, we attempted to determine the cleavage site of AACS. Mutagenesis analysis of AACS revealed that Asn547 is the specific cleavage site of AACS in mouse livers. The cleaved form of AACS (1-547) lost the ability to convert acetoacetate to acetoacetyl-CoA. Moreover, hydrodynamics-based gene transduction showed that overexpression of AACS (1-547) increases the protein expression of caveolin-1, the principal component of the caveolae. These results suggest that cleavage of AACS by legumain is critical for the regulation of enzymatic activity and results in gain-of-function changes.
Shinya Hasegawa, and
Daiki Inoue, and
Masahiro Yamasaki, and
Chuan Li, and
Masahiko Imai, and
Noriko Takahashi, and
Tetsuya Fukui
October 2014,
Biochemical and biophysical research communications,
Shinya Hasegawa, and
Daiki Inoue, and
Masahiro Yamasaki, and
Chuan Li, and
Masahiko Imai, and
Noriko Takahashi, and
Tetsuya Fukui
January 1985,
Methods in enzymology,
Shinya Hasegawa, and
Daiki Inoue, and
Masahiro Yamasaki, and
Chuan Li, and
Masahiko Imai, and
Noriko Takahashi, and
Tetsuya Fukui
April 1986,
Biochimica et biophysica acta,
Shinya Hasegawa, and
Daiki Inoue, and
Masahiro Yamasaki, and
Chuan Li, and
Masahiko Imai, and
Noriko Takahashi, and
Tetsuya Fukui
September 1985,
Analytical biochemistry,
Shinya Hasegawa, and
Daiki Inoue, and
Masahiro Yamasaki, and
Chuan Li, and
Masahiko Imai, and
Noriko Takahashi, and
Tetsuya Fukui
January 2018,
Biochemical and biophysical research communications,
Shinya Hasegawa, and
Daiki Inoue, and
Masahiro Yamasaki, and
Chuan Li, and
Masahiko Imai, and
Noriko Takahashi, and
Tetsuya Fukui
June 1984,
Cancer biochemistry biophysics,
Shinya Hasegawa, and
Daiki Inoue, and
Masahiro Yamasaki, and
Chuan Li, and
Masahiko Imai, and
Noriko Takahashi, and
Tetsuya Fukui
March 2010,
The Biochemical journal,
Shinya Hasegawa, and
Daiki Inoue, and
Masahiro Yamasaki, and
Chuan Li, and
Masahiko Imai, and
Noriko Takahashi, and
Tetsuya Fukui
March 1989,
The Biochemical journal,
Shinya Hasegawa, and
Daiki Inoue, and
Masahiro Yamasaki, and
Chuan Li, and
Masahiko Imai, and
Noriko Takahashi, and
Tetsuya Fukui
December 1987,
Biochimica et biophysica acta,
Shinya Hasegawa, and
Daiki Inoue, and
Masahiro Yamasaki, and
Chuan Li, and
Masahiko Imai, and
Noriko Takahashi, and
Tetsuya Fukui
October 2012,
Biochemical and biophysical research communications,
