-transparent.png){kind=logo}
The Modified Heparin-Binding L-Asparaginase of Wolinella succinogenes. 2016
E P Sannikova, and
N V Bulushova, and
S E Cheperegin, and
I I Gubaydullin, and
G G Chestukhina, and
V V Ryabichenko, and
I A Zalunin, and
E K Kotlova, and
G E Konstantinova, and
T S Kubasova, and
A A Shtil, and
V S Pokrovsky, and
S V Yarotsky, and
B D Efremov, and
D G Kozlov
State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545.
The modified asparaginase Was79 was derived from the recombinant wild-type L-asparaginase of Wolinella succinogenes. The Was79 contains the amino acid substitutions V23Q and K24T responsible for the resistance to trypsinolysis and the N-terminal heparin-binding peptide KRKKKGKGLGKKR responsible for the binding to heparin and tumor K562 cells in vitro. When tested on a mouse model of Fischer lymphadenosis L5178Y, therapeutic efficacy of Was79 was significantly higher than that of reference enzymes at all single therapeutic doses used (125-8000 IU/kg). At Was79 single doses of 500-8000 IU/kg, the complete remission rate of 100 % was observed. The Was79 variant can be expressed intracellularly in E. coli as a less immunogenic formyl-methionine-free form at high per cell production levels.
Related Publications
E P Sannikova, and
N V Bulushova, and
S E Cheperegin, and
I I Gubaydullin, and
G G Chestukhina, and
V V Ryabichenko, and
I A Zalunin, and
E K Kotlova, and
G E Konstantinova, and
T S Kubasova, and
A A Shtil, and
V S Pokrovsky, and
S V Yarotsky, and
B D Efremov, and
D G Kozlov
October 1996,
European journal of biochemistry,
E P Sannikova, and
N V Bulushova, and
S E Cheperegin, and
I I Gubaydullin, and
G G Chestukhina, and
V V Ryabichenko, and
I A Zalunin, and
E K Kotlova, and
G E Konstantinova, and
T S Kubasova, and
A A Shtil, and
V S Pokrovsky, and
S V Yarotsky, and
B D Efremov, and
D G Kozlov
August 1996,
Archives of microbiology,
E P Sannikova, and
N V Bulushova, and
S E Cheperegin, and
I I Gubaydullin, and
G G Chestukhina, and
V V Ryabichenko, and
I A Zalunin, and
E K Kotlova, and
G E Konstantinova, and
T S Kubasova, and
A A Shtil, and
V S Pokrovsky, and
S V Yarotsky, and
B D Efremov, and
D G Kozlov
January 2017,
Scientific reports,
E P Sannikova, and
N V Bulushova, and
S E Cheperegin, and
I I Gubaydullin, and
G G Chestukhina, and
V V Ryabichenko, and
I A Zalunin, and
E K Kotlova, and
G E Konstantinova, and
T S Kubasova, and
A A Shtil, and
V S Pokrovsky, and
S V Yarotsky, and
B D Efremov, and
D G Kozlov
January 1974,
Applied microbiology,
E P Sannikova, and
N V Bulushova, and
S E Cheperegin, and
I I Gubaydullin, and
G G Chestukhina, and
V V Ryabichenko, and
I A Zalunin, and
E K Kotlova, and
G E Konstantinova, and
T S Kubasova, and
A A Shtil, and
V S Pokrovsky, and
S V Yarotsky, and
B D Efremov, and
D G Kozlov
October 2001,
Archives of microbiology,
E P Sannikova, and
N V Bulushova, and
S E Cheperegin, and
I I Gubaydullin, and
G G Chestukhina, and
V V Ryabichenko, and
I A Zalunin, and
E K Kotlova, and
G E Konstantinova, and
T S Kubasova, and
A A Shtil, and
V S Pokrovsky, and
S V Yarotsky, and
B D Efremov, and
D G Kozlov
October 2007,
Antonie van Leeuwenhoek,
E P Sannikova, and
N V Bulushova, and
S E Cheperegin, and
I I Gubaydullin, and
G G Chestukhina, and
V V Ryabichenko, and
I A Zalunin, and
E K Kotlova, and
G E Konstantinova, and
T S Kubasova, and
A A Shtil, and
V S Pokrovsky, and
S V Yarotsky, and
B D Efremov, and
D G Kozlov
July 1985,
Journal of molecular biology,
E P Sannikova, and
N V Bulushova, and
S E Cheperegin, and
I I Gubaydullin, and
G G Chestukhina, and
V V Ryabichenko, and
I A Zalunin, and
E K Kotlova, and
G E Konstantinova, and
T S Kubasova, and
A A Shtil, and
V S Pokrovsky, and
S V Yarotsky, and
B D Efremov, and
D G Kozlov
May 1992,
European journal of biochemistry,
E P Sannikova, and
N V Bulushova, and
S E Cheperegin, and
I I Gubaydullin, and
G G Chestukhina, and
V V Ryabichenko, and
I A Zalunin, and
E K Kotlova, and
G E Konstantinova, and
T S Kubasova, and
A A Shtil, and
V S Pokrovsky, and
S V Yarotsky, and
B D Efremov, and
D G Kozlov
June 1993,
European journal of biochemistry,
E P Sannikova, and
N V Bulushova, and
S E Cheperegin, and
I I Gubaydullin, and
G G Chestukhina, and
V V Ryabichenko, and
I A Zalunin, and
E K Kotlova, and
G E Konstantinova, and
T S Kubasova, and
A A Shtil, and
V S Pokrovsky, and
S V Yarotsky, and
B D Efremov, and
D G Kozlov
January 1986,
The Biochemical journal,
