-transparent.png){kind=logo}
A cultured mouse myeloma cell line, S107, that secretes an IgA phosphocholine-binding immunoglobulin has been cloned in soft agar and overlaid with phosphocholine-hemocyanin. Spontaneous mutants that secrete immunoglobulin with a decreased ability to precipitate antigen were detected with this plate assay and occur at a very high frequency. From one such mutant, phenotypic revertants arise spontaneously with a frequency of 0.28-2.8%. This mutant and one of its revertants were studied, and they were found to differ from the parent and from each other serologically and in antigen binding. While it is not yet clear whether these findings bear any relationship to the normal generation of antibody diversity, they do indicate that it is possible to generate antigen binding diversity in somatic cells.
W D Cook, and
M D Scharff
January 1994,
Immunogenetics,
W D Cook, and
M D Scharff
January 1980,
Progress in clinical and biological research,
W D Cook, and
M D Scharff
December 1970,
Science (New York, N.Y.),
W D Cook, and
M D Scharff
January 1977,
Advances in immunology,
W D Cook, and
M D Scharff
December 1971,
Annals of the New York Academy of Sciences,
W D Cook, and
M D Scharff
March 1970,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
W D Cook, and
M D Scharff
January 1978,
The International journal of biochemistry,
W D Cook, and
M D Scharff
June 1975,
Transplantation proceedings,
W D Cook, and
M D Scharff
February 1971,
Biochemistry,
W D Cook, and
M D Scharff
April 2024,
Blood,
