BIOMAT Database Logo BIOMAT Database Logo

Characterization of the complete human elastin gene. Delineation of unusual features in the 5'-flanking region. 1989

M M Bashir, and Z Indik, and H Yeh, and N Ornstein-Goldstein, and J C Rosenbloom, and W Abrams, and M Fazio, and J Uitto, and J Rosenbloom
Department of Anatomy and Histology, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104.

Genomic clones encompassing the entire human elastin gene, including 11 kilobases flanking the ATG translation initiation codon, have been obtained and characterized by restriction enzyme analysis and extensive DNA sequencing. These analyses demonstrated that functionally distinct hydrophobic and cross-linking domains of the protein are segregated into separate exons throughout the gene. All exons are multiples of three nucleotides, and exon:intron borders always split codons in the same way which permits cassette-like alternative splicing. The 5'-flanking region lacks a canonical TATA sequence, is G + C-rich, and contains several SP1 binding sites and an AP2 binding site. Primer extension and S1 nuclease protection experiments indicate that transcription is initiated at multiple sites in the gene.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010920 Placenta A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES). Placentoma, Normal,Placentome,Placentas,Placentomes
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004549 Elastin alpha-Elastin,kappa-Elastin,alpha Elastin,kappa Elastin
D005260 Female Females
D005796 Genes A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms. Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D005809 Genes, Regulator Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions. Gene, Regulator,Regulator Gene,Regulator Genes,Regulatory Genes,Gene, Regulatory,Genes, Regulatory,Regulatory Gene
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein

Related Publications

M M Bashir, and Z Indik, and H Yeh, and N Ornstein-Goldstein, and J C Rosenbloom, and W Abrams, and M Fazio, and J Uitto, and J Rosenbloom
Deletion analyses of 5'-flanking region of the human elastin gene. Delineation of functional promoter and regulatory cis-elements.
June 1990, The Journal of biological chemistry,
M M Bashir, and Z Indik, and H Yeh, and N Ornstein-Goldstein, and J C Rosenbloom, and W Abrams, and M Fazio, and J Uitto, and J Rosenbloom
Characterization of the 5'-flanking region of the human preprogalanin gene.
April 1995, DNA and cell biology,
M M Bashir, and Z Indik, and H Yeh, and N Ornstein-Goldstein, and J C Rosenbloom, and W Abrams, and M Fazio, and J Uitto, and J Rosenbloom
Characterization of the 5'-flanking region of the human PTK6 gene.
April 2002, Biochimica et biophysica acta,
M M Bashir, and Z Indik, and H Yeh, and N Ornstein-Goldstein, and J C Rosenbloom, and W Abrams, and M Fazio, and J Uitto, and J Rosenbloom
Characterization of the 5' flanking region of the human MnSOD gene.
March 1996, Biochemical and biophysical research communications,
M M Bashir, and Z Indik, and H Yeh, and N Ornstein-Goldstein, and J C Rosenbloom, and W Abrams, and M Fazio, and J Uitto, and J Rosenbloom
Human glycogen debranching enzyme gene (AGL): complete structural organization and characterization of the 5' flanking region.
December 1996, Genomics,
M M Bashir, and Z Indik, and H Yeh, and N Ornstein-Goldstein, and J C Rosenbloom, and W Abrams, and M Fazio, and J Uitto, and J Rosenbloom
Characterization of the 5'-flanking region of the human dopamine transporter gene.
December 1999, Brain research. Molecular brain research,
M M Bashir, and Z Indik, and H Yeh, and N Ornstein-Goldstein, and J C Rosenbloom, and W Abrams, and M Fazio, and J Uitto, and J Rosenbloom
Characterization of the 5'-flanking region for the human fibrinogen beta gene.
February 1987, Nucleic acids research,
M M Bashir, and Z Indik, and H Yeh, and N Ornstein-Goldstein, and J C Rosenbloom, and W Abrams, and M Fazio, and J Uitto, and J Rosenbloom
Characterization of 5'-flanking region of human aggrecanase-1 (ADAMTS4) gene.
September 2000, Molecular biology reports,
M M Bashir, and Z Indik, and H Yeh, and N Ornstein-Goldstein, and J C Rosenbloom, and W Abrams, and M Fazio, and J Uitto, and J Rosenbloom
Molecular characterization of the 5'-flanking region of human genomic ETA gene.
January 1993, Biochemical and biophysical research communications,
M M Bashir, and Z Indik, and H Yeh, and N Ornstein-Goldstein, and J C Rosenbloom, and W Abrams, and M Fazio, and J Uitto, and J Rosenbloom
Regulation of elastin gene expression: evidence for functional promoter activity in the 5'-flanking region of the human gene.
February 1990, The Journal of investigative dermatology,
Copied contents to your clipboard!