Genotoxicity and immunotoxic effects of 1,2-dichloroethane in Wistar rats. 2016

Mohammad Iqbal Lone, and Nazia Nazam, and Aashiq Hussain, and Shashank K Singh, and Abid Hamid Dar, and Rauf Ahmad Najar, and Mohammed Hussein Al-Qahtani, and Waseem Ahmad
a Gene-Tox Laboratory, Division of Genetics, Department of Zoology, Aligarh Muslim University , Aligarh , UP , India.

Dichloroethane is widely used as a solvent, degreasing agent and in a variety of commercial products, and is known for being a ubiquitous contaminant in the environment. Important sources principally include the emissions from industrial processes, improper consumption, storage, and disposal methods. In view of the fact that the mechanism of its genotoxicity has not been satisfactorily elucidated, the acute in vivo toxicological impact is assessed in Rattus norvegicus. A systematic investigation has been made involving the use of conventional methods along with molecular and flow cytometric approaches. The micronucleus and chromosomal aberration frequencies were significantly elevated in bone marrow cells exposed to three concentrations at multiple treatment durations indicating positive time- and dose-response relationships. The mitotic index significantly decreased in similar concentrations in contrast to normal control. Separate studies were performed on blood cells for comet assay. It revealed dichloroethane-induced DNA damage in all exposures readily explainable in a dose- and time-dependent manner. Recent molecular techniques were further employed using leukocytes for the cell apoptosis/cycle and mitochondrial membrane potential employing propidium iodide staining and rhodamine-123, respectively. The effect on mitochondrial membrane permeability, cell cycle phases, and the DNA damage was analyzed through flow cytometry. These indicators revealed dichloroethane treatment decreased the mitochondrial membrane potential, affected the cell cycle, and confirmed the DNA damage, leading to apoptosis of the cells of the immune system responsible for immunotoxic effects of dichloroethane on rat leukocytes.

UI MeSH Term Description Entries
D007107 Immune System The body's defense mechanism against foreign organisms or substances and deviant native cells. It includes the humoral immune response and the cell-mediated response and consists of a complex of interrelated cellular, molecular, and genetic components. Immune Systems,System, Immune,Systems, Immune
D001854 Bone Marrow Cells Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells. Bone Marrow Cell,Cell, Bone Marrow,Cells, Bone Marrow,Marrow Cell, Bone,Marrow Cells, Bone
D002453 Cell Cycle The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE. Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002869 Chromosome Aberrations Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS. Autosome Abnormalities,Cytogenetic Aberrations,Abnormalities, Autosome,Abnormalities, Chromosomal,Abnormalities, Chromosome,Chromosomal Aberrations,Chromosome Abnormalities,Cytogenetic Abnormalities,Aberration, Chromosomal,Aberration, Chromosome,Aberration, Cytogenetic,Aberrations, Chromosomal,Aberrations, Chromosome,Aberrations, Cytogenetic,Abnormalities, Cytogenetic,Abnormality, Autosome,Abnormality, Chromosomal,Abnormality, Chromosome,Abnormality, Cytogenetic,Autosome Abnormality,Chromosomal Aberration,Chromosomal Abnormalities,Chromosomal Abnormality,Chromosome Aberration,Chromosome Abnormality,Cytogenetic Aberration,Cytogenetic Abnormality
D004249 DNA Damage Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS. DNA Injury,DNA Lesion,DNA Lesions,Genotoxic Stress,Stress, Genotoxic,Injury, DNA,DNA Injuries
D005025 Ethylene Dichlorides Toxic, chlorinated, saturated hydrocarbons. Include both the 1,1- and 1,2-dichloro isomers. The latter is considerably more toxic. It has a sweet taste, ethereal odor and has been used as a fumigant and intoxicant among sniffers. Has many household and industrial uses. Dichloroethanes,Dichlorides, Ethylene
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015386 Hazardous Substances Elements, compounds, mixtures, or solutions that are considered severely harmful to human health and the environment. They include substances that are toxic, corrosive, flammable, or explosive. Biohazard,Hazardous Chemical,Hazardous Chemicals,Hazardous Material,Hazardous Materials,Hazardous Substance,Toxic Environmental Substance,Toxic Substances, Environmental,Biohazards,Chemicals, Hazardous,Environmental Substances, Toxic,Toxic Environmental Substances,Chemical, Hazardous,Environmental Substance, Toxic,Environmental Toxic Substances,Material, Hazardous,Materials, Hazardous,Substance, Hazardous,Substance, Toxic Environmental,Substances, Environmental Toxic,Substances, Hazardous,Substances, Toxic Environmental
D017208 Rats, Wistar A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain. Wistar Rat,Rat, Wistar,Wistar Rats

Related Publications

Mohammad Iqbal Lone, and Nazia Nazam, and Aashiq Hussain, and Shashank K Singh, and Abid Hamid Dar, and Rauf Ahmad Najar, and Mohammed Hussein Al-Qahtani, and Waseem Ahmad
January 2011, Mutation research,
Mohammad Iqbal Lone, and Nazia Nazam, and Aashiq Hussain, and Shashank K Singh, and Abid Hamid Dar, and Rauf Ahmad Najar, and Mohammed Hussein Al-Qahtani, and Waseem Ahmad
May 2004, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
Mohammad Iqbal Lone, and Nazia Nazam, and Aashiq Hussain, and Shashank K Singh, and Abid Hamid Dar, and Rauf Ahmad Najar, and Mohammed Hussein Al-Qahtani, and Waseem Ahmad
November 1980, Mutation research,
Mohammad Iqbal Lone, and Nazia Nazam, and Aashiq Hussain, and Shashank K Singh, and Abid Hamid Dar, and Rauf Ahmad Najar, and Mohammed Hussein Al-Qahtani, and Waseem Ahmad
April 2014, Drug and chemical toxicology,
Mohammad Iqbal Lone, and Nazia Nazam, and Aashiq Hussain, and Shashank K Singh, and Abid Hamid Dar, and Rauf Ahmad Najar, and Mohammed Hussein Al-Qahtani, and Waseem Ahmad
January 1999, IARC monographs on the evaluation of carcinogenic risks to humans,
Mohammad Iqbal Lone, and Nazia Nazam, and Aashiq Hussain, and Shashank K Singh, and Abid Hamid Dar, and Rauf Ahmad Najar, and Mohammed Hussein Al-Qahtani, and Waseem Ahmad
January 1988, Reviews of environmental contamination and toxicology,
Mohammad Iqbal Lone, and Nazia Nazam, and Aashiq Hussain, and Shashank K Singh, and Abid Hamid Dar, and Rauf Ahmad Najar, and Mohammed Hussein Al-Qahtani, and Waseem Ahmad
January 2011, Report on carcinogens : carcinogen profiles,
Mohammad Iqbal Lone, and Nazia Nazam, and Aashiq Hussain, and Shashank K Singh, and Abid Hamid Dar, and Rauf Ahmad Najar, and Mohammed Hussein Al-Qahtani, and Waseem Ahmad
October 1979, IARC monographs on the evaluation of the carcinogenic risk of chemicals to humans,
Mohammad Iqbal Lone, and Nazia Nazam, and Aashiq Hussain, and Shashank K Singh, and Abid Hamid Dar, and Rauf Ahmad Najar, and Mohammed Hussein Al-Qahtani, and Waseem Ahmad
August 1985, Research communications in chemical pathology and pharmacology,
Mohammad Iqbal Lone, and Nazia Nazam, and Aashiq Hussain, and Shashank K Singh, and Abid Hamid Dar, and Rauf Ahmad Najar, and Mohammed Hussein Al-Qahtani, and Waseem Ahmad
February 2010, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
Copied contents to your clipboard!