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To elucidate the human placental L-lactate transport mechanism, we investigated L-lactate uptake by microvillous membrane vesicles (MMV) prepared from full termed human placenta using the rapid filtration technique. 1. The transport of L-lactate into the MMV was not dependent on the Na+ electrochemical gradient (extravesicular greater than intravesicular). 2. The transport of L-lactate into the MMV was dependent on the H+ gradient (extravesicular greater than intravesicular) and this H+ dependent L-lactate uptake showed a tendency to overshoot. This overshoot disappeared with the addition of H+ ionophore (FCCP). 3. The initial rate of this H+ dependent L-lactate transport into the MMV exhibited saturation kinetics with respect to the L-lactate concentration: An apparent Km of 4.35mM and Vmax of 2.78n mol/mg protein/20sec were calculated. These results indicated that there existed a H+ and L-lactate transport system in human placental MMV.
K Nabuchi, and
I Moriyama, and
M Akasaki, and
Y Katakami, and
H Hisanaga, and
Y Kato, and
M Ichijo
October 1987,
Nihon Sanka Fujinka Gakkai zasshi,
K Nabuchi, and
I Moriyama, and
M Akasaki, and
Y Katakami, and
H Hisanaga, and
Y Kato, and
M Ichijo
July 1985,
Nihon Sanka Fujinka Gakkai zasshi,
K Nabuchi, and
I Moriyama, and
M Akasaki, and
Y Katakami, and
H Hisanaga, and
Y Kato, and
M Ichijo
May 1987,
Nihon Sanka Fujinka Gakkai zasshi,
K Nabuchi, and
I Moriyama, and
M Akasaki, and
Y Katakami, and
H Hisanaga, and
Y Kato, and
M Ichijo
June 1987,
Nihon Sanka Fujinka Gakkai zasshi,
K Nabuchi, and
I Moriyama, and
M Akasaki, and
Y Katakami, and
H Hisanaga, and
Y Kato, and
M Ichijo
December 1985,
Nihon Sanka Fujinka Gakkai zasshi,
K Nabuchi, and
I Moriyama, and
M Akasaki, and
Y Katakami, and
H Hisanaga, and
Y Kato, and
M Ichijo
October 1986,
Nihon Sanka Fujinka Gakkai zasshi,
K Nabuchi, and
I Moriyama, and
M Akasaki, and
Y Katakami, and
H Hisanaga, and
Y Kato, and
M Ichijo
April 1986,
Nihon Sanka Fujinka Gakkai zasshi,
K Nabuchi, and
I Moriyama, and
M Akasaki, and
Y Katakami, and
H Hisanaga, and
Y Kato, and
M Ichijo
November 1993,
Biochimica et biophysica acta,
K Nabuchi, and
I Moriyama, and
M Akasaki, and
Y Katakami, and
H Hisanaga, and
Y Kato, and
M Ichijo
January 2007,
Placenta,
K Nabuchi, and
I Moriyama, and
M Akasaki, and
Y Katakami, and
H Hisanaga, and
Y Kato, and
M Ichijo
January 1992,
Placenta,
