Biomaterial Database

Detachment of oral bacteria from saliva-coated hydroxyapatite by human polymorphonuclear leukocytes. 1989

J C Erard, and K T Miyasaki, and L E Wolinsky

Section of Oral Biology, University of California, Los Angeles 90024-1668.

Human polymorphonuclear leukocytes (PMN) demonstrated the ability to detach Actinomyces viscosus, A. naeslundii and Streptococcus sanguis from saliva-coated hydroxyapatite beads (SHA). Between 60 to 80% of bacteria were detached within 1 hour at PMN-to-bacteria ratios between 1:10 to 1:22. Detachment was enhanced by treating bacteria with fresh but not heat-inactivated normal human serum. Detachment of serum-treated A. viscosus was inhibited by cytochalasin B, L-1-tosylamide-2-phenylethylchloromethyl ketone (TPCK), and deoxyglucose but not colchicine, phenylmethylsulfonyl fluoride (PMSF), N-carbobenzoxy-L-phenylalanine chloromethyl ketone (ZPCK), and sodium azide. In the absence of serum treatment, the detachment of A. viscosus was insensitive to lactose, galactose, and mannose. We conclude that PMN can efficiently detach bacteria from SHA, this detachment is enhanced by serum, and this enhancement is probably dependent upon complement. Additionally, detachment of A. viscosus bound to SHA by PMN (1) does not appear to involve bacterial lectin activity, (2) seems to be dependent upon glycolytic metabolism, microfilament formation, and the activity of a TPCK-sensitive serine protease, and (3) is not sensitive to inhibitors of tubulin polymerization or heme-protein activity.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008297	Male		Males
D009504	Neutrophils	Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.	LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D011480	Protease Inhibitors	Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).	Antiprotease,Endopeptidase Inhibitor,Endopeptidase Inhibitors,Peptidase Inhibitor,Peptidase Inhibitors,Peptide Hydrolase Inhibitor,Peptide Hydrolase Inhibitors,Peptide Peptidohydrolase Inhibitor,Peptide Peptidohydrolase Inhibitors,Protease Antagonist,Protease Antagonists,Antiproteases,Protease Inhibitor,Antagonist, Protease,Antagonists, Protease,Hydrolase Inhibitor, Peptide,Hydrolase Inhibitors, Peptide,Inhibitor, Endopeptidase,Inhibitor, Peptidase,Inhibitor, Peptide Hydrolase,Inhibitor, Peptide Peptidohydrolase,Inhibitor, Protease,Inhibitors, Endopeptidase,Inhibitors, Peptidase,Inhibitors, Peptide Hydrolase,Inhibitors, Peptide Peptidohydrolase,Inhibitors, Protease,Peptidohydrolase Inhibitor, Peptide,Peptidohydrolase Inhibitors, Peptide
D001769	Blood	The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
D003571	Cytochalasin B	A cytotoxic member of the CYTOCHALASINS.	Phomin
D003847	Deoxyglucose	2-Deoxy-D-arabino-hexose. An antimetabolite of glucose with antiviral activity.	2-Deoxy-D-glucose,2-Deoxyglucose,2-Desoxy-D-glucose,2 Deoxy D glucose,2 Deoxyglucose,2 Desoxy D glucose
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006882	Hydroxyapatites	A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed)	Hydroxyapatite Derivatives,Derivatives, Hydroxyapatite
D000190	Actinomyces	A genus of gram-positive, rod-shaped bacteria whose organisms are nonmotile. Filaments that may be present in certain species are either straight or wavy and may have swollen or clubbed heads.