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Four inhibitors of glycerol-3-phosphate dehydrogenase were isolated from Ginkgo biloba and identified as anacardic acids (6-tridecylsalicylic acid, 6-[(8Z)-pentadecenyl]salicylic acid, 6-[(9Z,12Z)-heptadecadienyl]salicylic acid, and 6-[(8Z)-heptadecenyl]salicylic acid) by instrumental analyses. Their 50% inhibitory concentrations against the enzyme were 1-3 μg/ml under the standard assay conditions. Anacardic acid inhibited the enzyme non-competitively. The sarcotesta contained most of anacardic acids, and nuts a little.
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