Acetylcholinesterase (AChE) distribution in the dorsal lateral geniculate nucleus (LGd) of 3 polyprotodont and 3 diprotodont marsupials has been compared with the cytoarchitecture and, as appropriate, with retinal terminal bands (RTBs) as demonstrated by tracers injected into the vitreous body. In this series those polyprotodont marsupials showing only partial segregation of retinal input (Virginia opossum and Tasmanian devil), display the least cell laminar differentiation. In them AChE staining is mainly diffuse but stronger in areas of greatest retinal terminal overlap. Segregation of visual input increases progressively in the remaining polyprotodont (eastern quoll) and in the diprotodonts (Tasmanian bettong, Tasmanian potoroo and brush-tailed possum), culminating in the last-named, in which segregation is most complete. Related to this development varying numbers of cell laminae/sublaminae appear and retinal terminal laminae/sublaminae (bands) are revealed. AChE activity can be correlated with both specific cell laminae/sublaminae and retinal terminal laminae/sublaminae in these complex LGds. Greatest AChE staining in every case is related to laminae/RTBs located in the medial part of the lateral (alpha) segment of LGd. Cytoarchitecturally, the ventral lateral geniculate nucleus (LGv), unlike the heterogeneous LGd, is remarkably homogeneous in the series of animals studied and shows well-stained AChE patches relating to areas receiving significant retinal input.