BIOMAT Database Logo BIOMAT Database Logo

Atomic force microscopy combined with optical microscopy for cells investigation. 2017

Mariafrancesca Cascione, and Valeria de Matteis, and Rosaria Rinaldi, and Stefano Leporatti
Dipartimento di Matematica e Fisica "Ennio De Giorgi", Università del Salento Via Monteroni, 73100, Lecce, Italy.

This review reports on the combined use of the atomic force microscopy (AFM) and several type of optical/fluorescence/laser scanning microscopy for investigating cells. It is shown that the hybrid systems of AFM with optical-derived microscopies enable to study in detail cell surface properties (such as topography), their mechanical properties (e.g., Young's modulus) mechanotransduction phenomena and allow to gain insight into biological-related pathways and mechanisms in the complex nanoworld of cells. Microsc. Res. Tech. 80:109-123, 2017. © 2016 Wiley Periodicals, Inc.

UI MeSH Term Description Entries
D007091 Image Processing, Computer-Assisted A technique of inputting two-dimensional or three-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer. Biomedical Image Processing,Computer-Assisted Image Processing,Digital Image Processing,Image Analysis, Computer-Assisted,Image Reconstruction,Medical Image Processing,Analysis, Computer-Assisted Image,Computer-Assisted Image Analysis,Computer Assisted Image Analysis,Computer Assisted Image Processing,Computer-Assisted Image Analyses,Image Analyses, Computer-Assisted,Image Analysis, Computer Assisted,Image Processing, Biomedical,Image Processing, Computer Assisted,Image Processing, Digital,Image Processing, Medical,Image Processings, Medical,Image Reconstructions,Medical Image Processings,Processing, Biomedical Image,Processing, Digital Image,Processing, Medical Image,Processings, Digital Image,Processings, Medical Image,Reconstruction, Image,Reconstructions, Image
D008853 Microscopy The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe. Compound Microscopy,Hand-Held Microscopy,Light Microscopy,Optical Microscopy,Simple Microscopy,Hand Held Microscopy,Microscopy, Compound,Microscopy, Hand-Held,Microscopy, Light,Microscopy, Optical,Microscopy, Simple
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D002468 Cell Physiological Phenomena Cellular processes, properties, and characteristics. Cell Physiological Processes,Cell Physiology,Cell Physiological Phenomenon,Cell Physiological Process,Physiology, Cell,Phenomena, Cell Physiological,Phenomenon, Cell Physiological,Physiological Process, Cell,Physiological Processes, Cell,Process, Cell Physiological,Processes, Cell Physiological
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013499 Surface Properties Characteristics or attributes of the outer boundaries of objects, including molecules. Properties, Surface,Property, Surface,Surface Property
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D018625 Microscopy, Atomic Force A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample. Atomic Force Microscopy,Force Microscopy,Scanning Force Microscopy,Atomic Force Microscopies,Force Microscopies,Force Microscopies, Scanning,Force Microscopy, Scanning,Microscopies, Atomic Force,Microscopies, Force,Microscopies, Scanning Force,Microscopy, Force,Microscopy, Scanning Force,Scanning Force Microscopies
D040542 Mechanotransduction, Cellular The process by which cells convert mechanical stimuli into a chemical response. It can occur in both cells specialized for sensing mechanical cues such as MECHANORECEPTORS, and in parenchymal cells whose primary function is not mechanosensory. Cellular Mechanotransduction,Mechanosensory Transduction,Signal Transduction, Mechanical,Mechanical Signal Transduction,Transduction, Mechanosensory

Related Publications

Mariafrancesca Cascione, and Valeria de Matteis, and Rosaria Rinaldi, and Stefano Leporatti
High-speed atomic force microscopy combined with inverted optical microscopy for studying cellular events.
January 2013, Scientific reports,
Mariafrancesca Cascione, and Valeria de Matteis, and Rosaria Rinaldi, and Stefano Leporatti
Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells.
May 2009, Nature methods,
Mariafrancesca Cascione, and Valeria de Matteis, and Rosaria Rinaldi, and Stefano Leporatti
Combined Traction Force-Atomic Force Microscopy Measurements of Neuronal Cells.
October 2022, Biomimetics (Basel, Switzerland),
Mariafrancesca Cascione, and Valeria de Matteis, and Rosaria Rinaldi, and Stefano Leporatti
Atomic Force Microscopy for Cell Membrane Investigation.
January 2019, Methods in molecular biology (Clifton, N.J.),
Mariafrancesca Cascione, and Valeria de Matteis, and Rosaria Rinaldi, and Stefano Leporatti
Combined atomic force microscopy and fluorescence microscopy.
January 2011, Methods in molecular biology (Clifton, N.J.),
Mariafrancesca Cascione, and Valeria de Matteis, and Rosaria Rinaldi, and Stefano Leporatti
Investigation of Red Blood Cells by Atomic Force Microscopy.
March 2022, Sensors (Basel, Switzerland),
Mariafrancesca Cascione, and Valeria de Matteis, and Rosaria Rinaldi, and Stefano Leporatti
Combined atomic force microscopy and optical microscopy measurements as a method to investigate particle uptake by cells.
March 2006, Small (Weinheim an der Bergstrasse, Germany),
Mariafrancesca Cascione, and Valeria de Matteis, and Rosaria Rinaldi, and Stefano Leporatti
Atomic force microscopy investigation of viruses.
January 2011, Methods in molecular biology (Clifton, N.J.),
Mariafrancesca Cascione, and Valeria de Matteis, and Rosaria Rinaldi, and Stefano Leporatti
Investigation of dialysis membranes with atomic force microscopy.
July 1992, Ultramicroscopy,
Mariafrancesca Cascione, and Valeria de Matteis, and Rosaria Rinaldi, and Stefano Leporatti
Microtubule Preparation for Investigation with High-Speed Atomic Force Microscopy.
January 2022, Methods in molecular biology (Clifton, N.J.),
Copied contents to your clipboard!