The purpose of the present study was to assess fitness and running performance in a group of recreational runners (men, n = 18; women, n = 13). 'Fitness' was determined on the basis of their physiological and metabolic responses during maximal and submaximal exercise. There were strong correlations between VO2 max and treadmill running speeds equivalent to blood lactate concentrations of 2 mmol l-1 (V-2 mM) or 4 mmol l-1 (V-4 mM), 'relative running economy' and 5 km times (r = -0.84), but modest and non-significant correlations between muscle fibre composition and running performance. The results of the submaximal exercise tests suggested that the female runners were as well trained as the male runners. However, the men still recorded faster 5 km times (19.20 +/- 1.97 min vs 20.97 +/- 1.70 min; P less than 0.05). Therefore the of the present study suggest that the faster performance times recorded by the men were best explained by their higher VO2 max values, rather than their training status per se.