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[Analysis of Monosaccharide Compositions in Paeoniae Radix Alba Polysaccharide]. 2015

Ya-dong Qin, and Rong-bin Wang, and Juan-juan Zhou, and Fei Li, and Xin Jin, and Qian Yang

OBJECTIVE To analyze the monosaccharide composition and the molar ratio of Paeoniae Radix Alba polysaccharide(PYPS). METHODS Pre-column derivation HPLC method was developed to determine the monosaccharide composition and the molar ratio of PYPS from Paeonia lactiflora root of different growth years and obtained by graded alcohol precipitation. RESULTS The results of HPLC analysis showed that PYPS consisted of Man, Rha, Glc, Ara, Gal and Xyl with the molar ratio of 156.26 : 0.73 : 0.85 : 1.03 : 1.17 : 0.79. Glc molar ratio increased gradually with the increasing of growth years, while the other five monosaccharides decreased. CONCLUSIONS Precolumn derivation HPLC method can be used to determine the monosaccharides in PYPS, molar ratio are differently in the different growth years and molecular weight of the PYPS, Glc is the main monosaccharide of PYPS.

UI MeSH Term Description Entries
D009005 Monosaccharides Single chain carbohydrates that are the most basic units of CARBOHYDRATES. They are typically colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. Monosaccharide,Simple Sugar,Simple Sugars,Sugar, Simple,Sugars, Simple
D011134 Polysaccharides Long chain polymeric CARBOHYDRATES composed of MONOSACCHARIDES linked by glycosidic bonds. Glycan,Glycans,Polysaccharide
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D018517 Plant Roots The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Plant Bulbs,Plant Root,Bulb, Plant,Bulbs, Plant,Plant Bulb,Root, Plant,Roots, Plant
D029596 Paeonia A plant genus of the family Paeoniaceae, order Dilleniales, subclass Dilleniidae, class Magnoliopsida. These perennial herbs are up to 2 m (6') tall. Leaves are alternate and are divided into three lobes, each lobe being further divided into three smaller lobes. The large flowers are symmetrical, bisexual, have 5 sepals, 5 petals (sometimes 10), and many stamens. Paeoniae Radix,Peony,Shakuyaku,Paeoniae Radices,Paeonias,Peonies,Radices, Paeoniae,Radix, Paeoniae,Shakuyakus

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