Biomaterial Database

Isolation of protein complexes from the model legume Medicago truncatula by tandem affinity purification in hairy root cultures. 2016

Jonas Goossens, and Nathan De Geyter, and Alan Walton, and Dominique Eeckhout, and Jan Mertens, and Jacob Pollier, and Jennifer Fiallos-Jurado, and Annick De Keyser, and Rebecca De Clercq, and Jelle Van Leene, and Kris Gevaert, and Geert De Jaeger, and Sofie Goormachtig, and Alain Goossens

Department of Plant Systems Biology, VIB, Technologiepark 927, B-9052, Gent, Belgium.

Tandem affinity purification coupled to mass spectrometry (TAP-MS) is one of the most powerful techniques to isolate protein complexes and elucidate protein interaction networks. Here, we describe the development of a TAP-MS strategy for the model legume Medicago truncatula, which is widely studied for its ability to produce valuable natural products and to engage in endosymbiotic interactions. As biological material, transgenic hairy roots, generated through Agrobacterium rhizogenes-mediated transformation of M. truncatula seedlings, were used. As proof of concept, proteins involved in the cell cycle, transcript processing and jasmonate signalling were chosen as bait proteins, resulting in a list of putative interactors, many of which confirm the interologue concept of protein interactions, and which can contribute to biological information about the functioning of these bait proteins in planta. Subsequently, binary protein-protein interactions among baits and preys, and among preys were confirmed by a systematic yeast two-hybrid screen. Together, by establishing a M. truncatula TAP-MS platform, we extended the molecular toolbox of this model species.

UI	MeSH Term	Description	Entries
D013559	Symbiosis	The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.	Endosymbiosis,Commensalism,Mutualism
D046913	Medicago truncatula	A plant species of the family FABACEAE used to study GENETICS because it is DIPLOID, self fertile, has a small genome, and short generation time.	Medicago truncatulas,truncatula, Medicago
D060054	Agrobacterium	A genus of gram negative, aerobic, rod-shaped bacteria found in soil, plants, and marine mud.
D018517	Plant Roots	The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)	Plant Bulbs,Plant Root,Bulb, Plant,Bulbs, Plant,Plant Bulb,Root, Plant,Roots, Plant
D030821	Plants, Genetically Modified	PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.	Genetically Modified Plants,Plants, Transgenic,Transgenic Plants,GMO Plants,Genetically Engineered Plants,Engineered Plant, Genetically,Engineered Plants, Genetically,GMO Plant,Genetically Engineered Plant,Genetically Modified Plant,Modified Plant, Genetically,Modified Plants, Genetically,Plant, GMO,Plant, Genetically Engineered,Plant, Genetically Modified,Plant, Transgenic,Plants, GMO,Plants, Genetically Engineered,Transgenic Plant